CAJ | 학술논문

目的：探讨毛细管电泳分析仪在诊断血红蛋白（Hb）CS-H病中的应用价值。方法用Sebia Capillar-ys 2型毛细管电泳仪分别对经地中海贫血基因分析确诊的34例Hb CS-H病患者和70例非Hb CS-H病患者（包括42例-SEA/-α3．7、19例-SEA/-α4．2、7例Hb WS-H、2例Hb QS-H）进行Hb电泳。采用缺口聚合酶链反应（Gap-PCR）和反向斑点杂交的方法对2组患者进行地中海贫血基因分析。结果毛细管电泳分析 Hb CS-H组的HbCS带含量为（1．89±1．33）％，HbH带含量为（1．07±0．86）％，HbA2带含量为（1．17±0．68）％；非Hb CS-H组均未能检测到HbCS带，有部分标本未检出HbH带，HbA2带含量为（1．81±1．21）％。2组HbA2带含量比较差异有统计学意义（P＜0．05）。毛细管电泳仪诊断Hb CS-H病的敏感性和特异性分别为88．2％和100．0％，阳性预测值为100．0％，阴性预测值为94．6％，诊断效率为96．2％，与地中海贫血基因分析比较差异无统计学意义（P＝0．134）。结论毛细管电泳可以用来快速诊断Hb CS-H病，能够为患者减轻部分经济负担。
목적：탐토모세관전영분석의재진단혈홍단백（Hb）CS-H병중적응용개치。방법용Sebia Capillar-ys 2형모세관전영의분별대경지중해빈혈기인분석학진적34례Hb CS-H병환자화70례비Hb CS-H병환자（포괄42례-SEA/-α3．7、19례-SEA/-α4．2、7례Hb WS-H、2례Hb QS-H）진행Hb전영。채용결구취합매련반응（Gap-PCR）화반향반점잡교적방법대2조환자진행지중해빈혈기인분석。결과모세관전영분석 Hb CS-H조적HbCS대함량위（1．89±1．33）％，HbH대함량위（1．07±0．86）％，HbA2대함량위（1．17±0．68）％；비Hb CS-H조균미능검측도HbCS대，유부분표본미검출HbH대，HbA2대함량위（1．81±1．21）％。2조HbA2대함량비교차이유통계학의의（P＜0．05）。모세관전영의진단Hb CS-H병적민감성화특이성분별위88．2％화100．0％，양성예측치위100．0％，음성예측치위94．6％，진단효솔위96．2％，여지중해빈혈기인분석비교차이무통계학의의（P＝0．134）。결론모세관전영가이용래쾌속진단Hb CS-H병，능구위환자감경부분경제부담。
Objective To investigate the application significance of capillary electrophoresis analyzer in the diagnosis of hemoglobin(Hb)CS-H disease.Methods A total of 34 patients with Hb CS-H disease confirmed by thalassemia genetic analysis and 70 patients with non Hb CS-H disease (including 42 cases of -SEA/-α3.7 ,1 9 cases of-SEA/-α4.2 ,7 cases of Hb WS-H and 2 cases of Hb QS-H)were determined for Hb with Sebia Capillarys 2 capillary electrophoresis.The thalassemia genetic analysis of the 2 groups was conducted by gap-polymerase chain reaction (Gap-PCR)and reverse dot blot hybridization.Results The HbCS and HbH contents of Hb CS-H group were (1 .89 ± 1 .33)% and (1 .07 ±0.86)%,and the HbA2 content was (1 .1 7 ±0.68)%.However,the samples with HbCS and a part of HbH were not detected in non-Hb CS-H group,and the HbA2 content was (1 .81 ±1 .21 )%.Compared with Hb CS-H group and non-Hb CS-H group for HbA2 ,there were significant significance (P<0.05 ).Compared with the thalassemia genetic analysis,the sensitivity of capillary electrophoresis for the diagnosis of Hb CS-H disease was 88.2%, the specificity was 1 00.0%,the positive predictive value was 1 00.0%,the negative predictive value was 94.6%,the diagnosis efficiency was 96.2%,and there was no statistical significance (P=0.1 34).Conclusions The capillary electrophoresis can be used to the rapid diagnosis of Hb CS-H disease,and it will alleviate some of the economic burdens for patients.