CAJ | 학술논문

为了探究印楝细胞培养物控制害虫的效果，本文通过诱导幼叶、继代培养获得印楝的松散型愈伤组织，再经振荡培养获得悬浮细胞培养物；然后采用二氯甲烷溶剂浸提愈伤组织、悬浮培养物及悬浮培养细胞，制备相应的浸提液；用二氯甲烷分别将这些浸提液稀释成含0.003%印楝素A 的控虫工作液，用其分别处理黄粉虫幼虫，在12,d内，考察其控虫效果．结果表明：3种浸提液的致死效应明显高于阴性对照，其中，第9天的最大致死率达到20%，但它们要明显低于0.003%印楝素乳油农药的处理；3种浸提液对幼虫的虫体质量、拒食和趋避作用等方面效果较好，均达到印楝乳油杀虫剂的控虫水平．印楝细胞培养物经一步浸提后，直接用于控虫制剂的生产，可以节约分离、提纯印楝素等生产性成本．
위료탐구인련세포배양물공제해충적효과，본문통과유도유협、계대배양획득인련적송산형유상조직，재경진탕배양획득현부세포배양물；연후채용이록갑완용제침제유상조직、현부배양물급현부배양세포，제비상응적침제액；용이록갑완분별장저사침제액희석성함0.003%인련소A 적공충공작액，용기분별처리황분충유충，재12,d내，고찰기공충효과．결과표명：3충침제액적치사효응명현고우음성대조，기중，제9천적최대치사솔체도20%，단타문요명현저우0.003%인련소유유농약적처리；3충침제액대유충적충체질량、거식화추피작용등방면효과교호，균체도인련유유살충제적공충수평．인련세포배양물경일보침제후，직접용우공충제제적생산，가이절약분리、제순인련소등생산성성본．
To explore the pest control effect of neem cultures,relaxed callus was induced and subcultured from young neem leaves,from which suspension cells were obtained by shaking the culture. The callus,suspension cultures and suspension cells were turned into insecticidal solution by using dichloromethane dissolvent.The three kinds of solution were later di-luted into their working solutions with 0.003%Azadirachtin by using dichloromethane;and then those solutions were applied to Tenebrio molitor larvae,one by one. In 12,days,their pest control effects were observed. The results indicated that all the three kinds of dichloromethane extracts had a lethal rate against Tenebrio molitor larvae higher than that of the negative con-trol. The maximum lethal rate of the extracts reached 20%when cultured for 9,days,but the lethal rate was lower than that of 0.003%Azadirachtin EC. The extracts all gave good performance in dealing with the body weight,antifeeding and repellent rate of the larvae,and they all reached the pesticide control level of Azadirachtin Emulsion. In conclusion,neem cell cultures,by one step extraction,can be directly used as pest control agent.This method can lower the production cost of Azadirachtin separation and purification.