CAJ | 학술논문

目的：研究 MAPK/ ERK 通路及泛素连接酶 c - Cbl 调控埃克替尼抑制 eCC827细胞增殖和诱导细胞凋亡的作用机制。方法：采用 MTT 法检测 eCC827对埃克替尼的敏感性，Annexin V - PI 流式细胞术检测细胞凋亡，Western blot 检测相关蛋白表达，SPSS 18.0进行统计学分析。结果：埃克替尼处理 eCC827细胞24h 的IC50为155.6μmol/ L。埃克替尼诱导 eCC827细胞早期凋亡并具有剂量依赖性。Western blot 结果显示埃克替尼可诱导 P - EGFR 和 P - ERK 下调并引起 c - Cbl 下调。结论：埃克替尼能够明显抑制 eCC827细胞增殖，诱导细胞早期凋亡。其机制可能与 c - Cbl 参与的 P - EGFR 和 P - ERK 蛋白表达水平下调，从而抑制细胞增殖通路活化有关。
목적：연구 MAPK/ ERK 통로급범소련접매 c - Cbl 조공애극체니억제 eCC827세포증식화유도세포조망적작용궤제。방법：채용 MTT 법검측 eCC827대애극체니적민감성，Annexin V - PI 류식세포술검측세포조망，Western blot 검측상관단백표체，SPSS 18.0진행통계학분석。결과：애극체니처리 eCC827세포24h 적IC50위155.6μmol/ L。애극체니유도 eCC827세포조기조망병구유제량의뢰성。Western blot 결과현시애극체니가유도 P - EGFR 화 P - ERK 하조병인기 c - Cbl 하조。결론：애극체니능구명현억제 eCC827세포증식，유도세포조기조망。기궤제가능여 c - Cbl 삼여적 P - EGFR 화 P - ERK 단백표체수평하조，종이억제세포증식통로활화유관。
Objective:To investigate the role of c - Cbl involved in inactivation of MAPK/ ERK Pathway induced by Icotinib. Methods:The effect of Icotinib on the Proliferation of eCC827 was detected by MTT. Cell aPoPtosis was determined by flow cytometry with Annexin V - PI staining. Protein exPression levels were detected by Western blot. All exPerimental data was analyzed with SPSS 18. 0 software. Results:The IC50 value of eCC827 incuabted with Ico-tinib for 24h was 155. 6 μmol/ L;Icotinib induced the aPoPtosis of eCC827 in a dose - dePendent manner. Western blot results showed that Icotinib decreased the exPression level of P - EGFR,P - ERK and c - Cbl. Conclusion:Ico-tinib significantly inhibits eCC827 Proliferation by inducing aPoPtosis. The reason is that c - Cbl leads to inactivation of MAPK/ ERK Pathway as showed by downregualtion of P - EGFR and P - ERK.