分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2013年
6期
394-397
,共4页
人巨细胞病毒%IgM%IgG%IgG亲合力%原发性感染
人巨細胞病毒%IgM%IgG%IgG親閤力%原髮性感染
인거세포병독%IgM%IgG%IgG친합력%원발성감염
Human cytomegalovirus%IgM%IgG%IgG avidity%Primary Infection
目的：探讨血清人巨细胞病毒（HCMV）IgM水平在低龄儿童中诊断原发性HCMV感染的意义。方法收集临床诊断为HCMV感染的患儿血标本56例，年龄为0.5~1岁，采用全自动化学发光法分别定量检测血清HCMV IgG、IgM抗体水平，以及IgG亲和力指数。结果 HCMV IgG+患儿亲和力检测结果发现，其中IgM-/IgG+的5例患者都为高亲和力IgG，而IgM+/IgG+的42例患者中25例是低亲和力IgG，占59.5%。根据IgM定量检测结果进一步将此47例HCMV IgG+患儿由低至高分成5组，分别为IgM阴性组（<30 AU/mL），30~100 AU/mL组，100~170 AU/mL组，170~240 AU/mL组以及大于240 AU/mL组。随着IgM抗体水平的增加，低亲和力样本所占比例呈增高趋势，经卡方检验，差异有统计学意义（χ2=12.72，P<0.05）。结论在0.5~1岁婴儿中，HCMV IgM水平与HCMV原发感染呈正相关，临床同时参考IgM定量检测和HCMV IgG亲合力检测结果，将对0.5~1岁以内婴幼儿近期HCMV感染发生时间的判定起到有益的互补作用。先进行HCMV IgM定量检测，再进行阳性病例的IgG亲和力检测是低龄儿童原发HCMV感染实验室诊断的可行方案。
目的：探討血清人巨細胞病毒（HCMV）IgM水平在低齡兒童中診斷原髮性HCMV感染的意義。方法收集臨床診斷為HCMV感染的患兒血標本56例，年齡為0.5~1歲，採用全自動化學髮光法分彆定量檢測血清HCMV IgG、IgM抗體水平，以及IgG親和力指數。結果 HCMV IgG+患兒親和力檢測結果髮現，其中IgM-/IgG+的5例患者都為高親和力IgG，而IgM+/IgG+的42例患者中25例是低親和力IgG，佔59.5%。根據IgM定量檢測結果進一步將此47例HCMV IgG+患兒由低至高分成5組，分彆為IgM陰性組（<30 AU/mL），30~100 AU/mL組，100~170 AU/mL組，170~240 AU/mL組以及大于240 AU/mL組。隨著IgM抗體水平的增加，低親和力樣本所佔比例呈增高趨勢，經卡方檢驗，差異有統計學意義（χ2=12.72，P<0.05）。結論在0.5~1歲嬰兒中，HCMV IgM水平與HCMV原髮感染呈正相關，臨床同時參攷IgM定量檢測和HCMV IgG親閤力檢測結果，將對0.5~1歲以內嬰幼兒近期HCMV感染髮生時間的判定起到有益的互補作用。先進行HCMV IgM定量檢測，再進行暘性病例的IgG親和力檢測是低齡兒童原髮HCMV感染實驗室診斷的可行方案。
목적：탐토혈청인거세포병독（HCMV）IgM수평재저령인동중진단원발성HCMV감염적의의。방법수집림상진단위HCMV감염적환인혈표본56례，년령위0.5~1세，채용전자동화학발광법분별정량검측혈청HCMV IgG、IgM항체수평，이급IgG친화력지수。결과 HCMV IgG+환인친화력검측결과발현，기중IgM-/IgG+적5례환자도위고친화력IgG，이IgM+/IgG+적42례환자중25례시저친화력IgG，점59.5%。근거IgM정량검측결과진일보장차47례HCMV IgG+환인유저지고분성5조，분별위IgM음성조（<30 AU/mL），30~100 AU/mL조，100~170 AU/mL조，170~240 AU/mL조이급대우240 AU/mL조。수착IgM항체수평적증가，저친화력양본소점비례정증고추세，경잡방검험，차이유통계학의의（χ2=12.72，P<0.05）。결론재0.5~1세영인중，HCMV IgM수평여HCMV원발감염정정상관，림상동시삼고IgM정량검측화HCMV IgG친합력검측결과，장대0.5~1세이내영유인근기HCMV감염발생시간적판정기도유익적호보작용。선진행HCMV IgM정량검측，재진행양성병례적IgG친화력검측시저령인동원발HCMV감염실험실진단적가행방안。
Objective To study the diagnostic role of human cytomegalovirus (HCMV) IgM level for HCMV primary infection in lower aged children. Methods 56 serum samples were collected from 0.5~1 years old new borns, who were clinically diagnosed as HCMV infection. HCMV IgM/IgG and IgG avidity were quantitatively detected by automatic CLIA. Results Among the HCMV IgG positive samples, 5 samples, which were IgM-/IgG+, showed high HCMV IgG avidities. In the remaining 42 samples, which were IgM+/IgG+, 25 samples (about 59.5%) showed low IgG avidities. Based on the HCMV IgM level, the samples were further divided into 5 groups:IgM negative group (<30 AU/mL), 30~100 AU/mL group, 100~170 AU/mL group, 170~240 AU/mL group and >240 AU/mL group. It was found that the percentage of HCMV IgG low avidity samples were increased with increases of IgM levels. The difference was statistically significant (χ2=12.72, P<0.05). Conclusion High HCMV IgM level in serum are positively correlated to HCMV primary infection among newborns of 0.5~1 age. Quantitative IgM assay could be complimentary to diagnosis of primary HCMV infection in paralle with IgG avidity test. This data imply that quantitatively detection of HCMV IgM antibody followed by IgG avidity detection is practical proposal for laboratory diagnosis of HCMV primary infection in lower aged children.
