分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2013年
6期
388-393
,共6页
何展明%马蒙蒙%陈清%谭炳炎
何展明%馬矇矇%陳清%譚炳炎
하전명%마몽몽%진청%담병염
邻苯二甲酸二丁酯%青春期女生%血清%气相色谱-质谱法
鄰苯二甲痠二丁酯%青春期女生%血清%氣相色譜-質譜法
린분이갑산이정지%청춘기녀생%혈청%기상색보-질보법
Di-n-butyl phthalate%Puberty girls%Serum%Gas chromatography-mass spectrometry
目的：了解广州市发育正常青春期女生（12~15岁）血清中邻苯二甲酸二丁酯（DBP）暴露水平，并初步探讨其与初潮年龄的关系。方法2012年3月，选择广州市白云区某中学30名健康体检的女生作为研究对象，采集静脉血样本，利用气相色谱-质谱联用（GC-MS）分析方法检测其血清中DBP水平，以广东地区汉族女孩2005年初潮年龄的均值12.4岁为准，将调查对象分为初潮年龄<12.4岁组和初潮年龄≥12.4岁组，比较不同初潮年龄组女生血清DBP检出率及浓度分布的差异。结果30例女生的血样中，19例可检出DBP，检出率为63.3%，DBP的浓度范围为0~740μg/L，浓度中位数为60μg/L，且无年龄差异，其中初潮年龄<12.4岁组检出率为75.0%，初潮年龄≥12.4岁组检出率为86.4%，且前者血清DBP浓度分布的平均秩次高于后者，但两组检出率及浓度分布差异均无统计学意义。结论30名发育正常青春期女生血清DBP检出率和浓度中位数均处于较高水平，但无年龄差异，尚不能说明青春期女生血清DBP检出率及浓度分布与初潮年龄的相关性，但值得进一步加大样本量研究。
目的：瞭解廣州市髮育正常青春期女生（12~15歲）血清中鄰苯二甲痠二丁酯（DBP）暴露水平，併初步探討其與初潮年齡的關繫。方法2012年3月，選擇廣州市白雲區某中學30名健康體檢的女生作為研究對象，採集靜脈血樣本，利用氣相色譜-質譜聯用（GC-MS）分析方法檢測其血清中DBP水平，以廣東地區漢族女孩2005年初潮年齡的均值12.4歲為準，將調查對象分為初潮年齡<12.4歲組和初潮年齡≥12.4歲組，比較不同初潮年齡組女生血清DBP檢齣率及濃度分佈的差異。結果30例女生的血樣中，19例可檢齣DBP，檢齣率為63.3%，DBP的濃度範圍為0~740μg/L，濃度中位數為60μg/L，且無年齡差異，其中初潮年齡<12.4歲組檢齣率為75.0%，初潮年齡≥12.4歲組檢齣率為86.4%，且前者血清DBP濃度分佈的平均秩次高于後者，但兩組檢齣率及濃度分佈差異均無統計學意義。結論30名髮育正常青春期女生血清DBP檢齣率和濃度中位數均處于較高水平，但無年齡差異，尚不能說明青春期女生血清DBP檢齣率及濃度分佈與初潮年齡的相關性，但值得進一步加大樣本量研究。
목적：료해엄주시발육정상청춘기녀생（12~15세）혈청중린분이갑산이정지（DBP）폭로수평，병초보탐토기여초조년령적관계。방법2012년3월，선택엄주시백운구모중학30명건강체검적녀생작위연구대상，채집정맥혈양본，이용기상색보-질보련용（GC-MS）분석방법검측기혈청중DBP수평，이엄동지구한족녀해2005년초조년령적균치12.4세위준，장조사대상분위초조년령<12.4세조화초조년령≥12.4세조，비교불동초조년령조녀생혈청DBP검출솔급농도분포적차이。결과30례녀생적혈양중，19례가검출DBP，검출솔위63.3%，DBP적농도범위위0~740μg/L，농도중위수위60μg/L，차무년령차이，기중초조년령<12.4세조검출솔위75.0%，초조년령≥12.4세조검출솔위86.4%，차전자혈청DBP농도분포적평균질차고우후자，단량조검출솔급농도분포차이균무통계학의의。결론30명발육정상청춘기녀생혈청DBP검출솔화농도중위수균처우교고수평，단무년령차이，상불능설명청춘기녀생혈청DBP검출솔급농도분포여초조년령적상관성，단치득진일보가대양본량연구。
Objective To determine the exposure level of serum di-n-butyl phthalate (DBP) in healthy puberty girls aged 12 to 15 years in Guangzhou and study the relationship between DBP exposure and menarche age. Methods The serum samples were collected intravenously from 30 puberty girls who were identified as normal in their school physical examination in March 2012 in Baiyun District of Guangzhou City, aged 12 to 15. For serum DBP level determination, gas chromatography-mass spectrometry method was established. Girls were divided into two groups using menarche age 12.4 which was the mean of the Guangdong Han nationality girls in 2005 as a cut-point, one was the group with menache age early than 12.4 and the rest belonged to the other one. Then detection rate and concentration distribution of DBP between the two groups were analyzed. Results <br> DBP could be detected in 19 serum samples from total 30 samples and the rate of detection was 63.3%. The concentrations of DBP ranged from non detectable to 740μg/L with the median of 60μg/L while no variation was found by age. The rate of detection in group with menache age early than 12.4 was 75%while the other one was86.4% and the former had a higher mean rank of DBP concentration. However, the differences showed no statistical significance between the two group (P=0.672 and P=0.962, respectively). Conclusion The detection rate and serum median concentration of DBP of the 30 normal puberty girls were both in high level with no variation by age and no association was found between serum DBP exposure and menarche age in puberty girls.
