分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2013年
6期
371-373
,共3页
汪芸%王书芹%周小军%王静%毕超%禤瑞霞%陈钰炜%卢金文%包贵贤
汪蕓%王書芹%週小軍%王靜%畢超%禤瑞霞%陳鈺煒%盧金文%包貴賢
왕예%왕서근%주소군%왕정%필초%훤서하%진옥위%로금문%포귀현
鼻咽癌%癌前病变%Cul-1%Skp2
鼻嚥癌%癌前病變%Cul-1%Skp2
비인암%암전병변%Cul-1%Skp2
Nasopharyngeal carcinoma%Precancerous lesion%Cullin-1%Skp2
目的：探讨细胞周期蛋白Cullin-1（Cul-1）和S期激酶相关蛋白2（Skp2）在鼻咽癌及癌前病变组织中的表达及意义，同时对比它们在鼻咽癌及癌前病变组织中的表达水平，比较二者的分布情况，找出其差异性及相关关系。方法应用免疫组化方法检测Cul-1和Skp2蛋白在20例分化型癌、20例未分化癌和20例鼻咽癌前病变中的表达。结果在20例分化型癌、20例未分化癌和20例鼻咽癌前病变中Cul-1的平均阳性表达率分别为76%、77%、54%；阳性表达值分别为1.63±0.7、1.62±0.68、1.15±0.64， Cul-1在2种鼻咽癌中的表达高于癌前病变，差异有统计学意义（P<0.05），2种鼻咽癌之间无差异；Skp2在20例分化型癌、20例未分化癌和20例鼻咽癌前病变中的平均阳性表达率为32%、41%、33%，平均阳性表达值分别为0.53±0.49、0.82±0.54、0.72±0.43，无统计学差异。对20例分化型鼻咽癌标本Cul-1、Skp2阳性表达值进行相关关系分析，两者相关性不显著。结论 Cul-1参与了鼻咽癌发生、发展，Skp2则可能参与了鼻咽癌的发生过程，它参与的细胞周期调节可能是鼻咽癌发生的早期事件；两种蛋白因子在细胞周期调控中的关系有待进一步验证。
目的：探討細胞週期蛋白Cullin-1（Cul-1）和S期激酶相關蛋白2（Skp2）在鼻嚥癌及癌前病變組織中的錶達及意義，同時對比它們在鼻嚥癌及癌前病變組織中的錶達水平，比較二者的分佈情況，找齣其差異性及相關關繫。方法應用免疫組化方法檢測Cul-1和Skp2蛋白在20例分化型癌、20例未分化癌和20例鼻嚥癌前病變中的錶達。結果在20例分化型癌、20例未分化癌和20例鼻嚥癌前病變中Cul-1的平均暘性錶達率分彆為76%、77%、54%；暘性錶達值分彆為1.63±0.7、1.62±0.68、1.15±0.64， Cul-1在2種鼻嚥癌中的錶達高于癌前病變，差異有統計學意義（P<0.05），2種鼻嚥癌之間無差異；Skp2在20例分化型癌、20例未分化癌和20例鼻嚥癌前病變中的平均暘性錶達率為32%、41%、33%，平均暘性錶達值分彆為0.53±0.49、0.82±0.54、0.72±0.43，無統計學差異。對20例分化型鼻嚥癌標本Cul-1、Skp2暘性錶達值進行相關關繫分析，兩者相關性不顯著。結論 Cul-1參與瞭鼻嚥癌髮生、髮展，Skp2則可能參與瞭鼻嚥癌的髮生過程，它參與的細胞週期調節可能是鼻嚥癌髮生的早期事件；兩種蛋白因子在細胞週期調控中的關繫有待進一步驗證。
목적：탐토세포주기단백Cullin-1（Cul-1）화S기격매상관단백2（Skp2）재비인암급암전병변조직중적표체급의의，동시대비타문재비인암급암전병변조직중적표체수평，비교이자적분포정황，조출기차이성급상관관계。방법응용면역조화방법검측Cul-1화Skp2단백재20례분화형암、20례미분화암화20례비인암전병변중적표체。결과재20례분화형암、20례미분화암화20례비인암전병변중Cul-1적평균양성표체솔분별위76%、77%、54%；양성표체치분별위1.63±0.7、1.62±0.68、1.15±0.64， Cul-1재2충비인암중적표체고우암전병변，차이유통계학의의（P<0.05），2충비인암지간무차이；Skp2재20례분화형암、20례미분화암화20례비인암전병변중적평균양성표체솔위32%、41%、33%，평균양성표체치분별위0.53±0.49、0.82±0.54、0.72±0.43，무통계학차이。대20례분화형비인암표본Cul-1、Skp2양성표체치진행상관관계분석，량자상관성불현저。결론 Cul-1삼여료비인암발생、발전，Skp2칙가능삼여료비인암적발생과정，타삼여적세포주기조절가능시비인암발생적조기사건；량충단백인자재세포주기조공중적관계유대진일보험증。
Objective To detect the expression of Cullin-1 (Cul-1) and S-phase kinase associated protein 2 (Skp2) in the tissues of nasopharyngeal carcinoma (NPC) and precancerous lesion, and elucidate the relationship between them. Methods Immunohistoehemical methods were taken to detect the expression of Cul-1 and Skp2 in the tissues taken before treatment among 20 differentiated carcinoma patients, 20 undifferentiated carcinoma patients, and 20 precancerous lesion patients. At last, the relationship between the expression of these protein was analyzed. Results The positive expression rates of Cul-1 in the tissues of differentiated carcinoma, undifferentiated carcinoma and precancerous lesion were 76%, 77% and 54%, respectively. The expression value were 1.63±0.7, 1.62±0.68, 1.15±0.64, respectively. The differences between differentiated carcinoma and precancerous lesion, undifferentiated carcinoma and precancerous lesion were statistically significant (P<0.05). The positive expression rates of Skp2 in the tissues of differentiated carcinoma, undifferentiated carcinoma and precancerous lesion were 32%, 41%, 33%, respectively. The expression value were 0.53±0.49, 0.82±0.54, 0.72±0.43, and there were no difference between them. To analyze the expression of Cul-1 and Skp2 in 20 differentiated carcinoma, which had no significant correlation. Conclusion Cul-1 and Skp2 protein were involved in the occurrence and development of human nasopharyngeal carcinoma. And the Skp2 participated in cell cycle regulation that may be an early event of nasopharyngeal carcinoma. The relationship between Cul-1 and Skp2 in cell cycle regulation need further validation.
