CAJ | 학술논문

目的观察晚期糖基化终产物（AGEs）对足细胞α-actinin-4的影响及氯沙坦的干预作用。方法不同浓度的AGEs（0,20,40,80μg/mL）干预足细胞24 h后，采用免疫印迹法检测足细胞α-actinin-4的表达。通过激光共聚焦显微镜观察足细胞肌动蛋白F-actin和α-actinin-4的分布，并在氯沙坦预处理足细胞后，观察α-actinin-4和F-actin的改变。结果与对照组相比，AGEs（80μg/mL）可明显降低足细胞α-actinin-4的表达[（62±13）%vs.100%，P<0.05]，使α-actinin-4由膜周分布转为核周分布，并使肌动蛋白F-actin发生重构，而氯沙坦预处理可减轻AGEs介导的α-actinin-4的下调[（80±14）%vs.（62±13）%，P<0.05]和α-actinin-4和F-actin的重构，P<0.05。结论 AGEs可下调α-actinin-4表达，介导骨架蛋白的重构，而氯沙坦可减轻AGEs介导的这些改变。
목적관찰만기당기화종산물（AGEs）대족세포α-actinin-4적영향급록사탄적간예작용。방법불동농도적AGEs（0,20,40,80μg/mL）간예족세포24 h후，채용면역인적법검측족세포α-actinin-4적표체。통과격광공취초현미경관찰족세포기동단백F-actin화α-actinin-4적분포，병재록사탄예처리족세포후，관찰α-actinin-4화F-actin적개변。결과여대조조상비，AGEs（80μg/mL）가명현강저족세포α-actinin-4적표체[（62±13）%vs.100%，P<0.05]，사α-actinin-4유막주분포전위핵주분포，병사기동단백F-actin발생중구，이록사탄예처리가감경AGEs개도적α-actinin-4적하조[（80±14）%vs.（62±13）%，P<0.05]화α-actinin-4화F-actin적중구，P<0.05。결론 AGEs가하조α-actinin-4표체，개도골가단백적중구，이록사탄가감경AGEs개도적저사개변。
Objective To investigate the effects of advanced glycation end-products (AGEs) on the expression of α-actinin-4 and their mechanism. Methods Podocytes were incubated with different concentrations of AGEs for 24 hours, and the expression levels of α-actinin-4 were measured by western blot. The arrangement of α-actinin-4 and F-actin were observed by laser scanning confocal microscopy. Then podocytes were pretreated with losartan for 60 min before AGEs added. The changes of α-actinin-4 and cytoskeleton were observed. Results Incubation with AGEs (80μg/mL) resulted in a significant decrease in the expression ofα-actinin-4 [(62±13)%vs. 100%, P<0.05], AGEs (80μg/mL) also induced bothα-actinin-4 and F-actin rearrangement. However, pretreatment with losartan (100 μmol/L) prevented the effects induced by AGEs in podocytes. Conclusion AGEs downregulate α-actinin-4 expression and induce cytoskeleton reorganization, and losartan prevent the changes induced by AGEs.