中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
588-589
,共2页
毛细管柱气相色谱法%顶空进样%残留溶剂
毛細管柱氣相色譜法%頂空進樣%殘留溶劑
모세관주기상색보법%정공진양%잔류용제
Capillary GC%Headspace injection%Residual organic solvents
目的：建立毛细管柱气相色谱法测定紫杉醇中残留的有机溶剂。方法：采用HP-INNOWAX毛细管色谱柱、顶空进样、氢火焰离子化检测器,进样口温度为250℃,检测器温度为300℃,柱温为35℃保持5 min,再以25℃·min-1升至260℃。结果：正己烷、丙酮、乙酸乙酯与甲醇相邻峰间的分离度及检测灵敏度均符合要求。结论：该法简便、快速、灵敏,结果准确可靠,可用于紫杉醇中残留有机溶剂的测定。
目的：建立毛細管柱氣相色譜法測定紫杉醇中殘留的有機溶劑。方法：採用HP-INNOWAX毛細管色譜柱、頂空進樣、氫火燄離子化檢測器,進樣口溫度為250℃,檢測器溫度為300℃,柱溫為35℃保持5 min,再以25℃·min-1升至260℃。結果：正己烷、丙酮、乙痠乙酯與甲醇相鄰峰間的分離度及檢測靈敏度均符閤要求。結論：該法簡便、快速、靈敏,結果準確可靠,可用于紫杉醇中殘留有機溶劑的測定。
목적：건립모세관주기상색보법측정자삼순중잔류적유궤용제。방법：채용HP-INNOWAX모세관색보주、정공진양、경화염리자화검측기,진양구온도위250℃,검측기온도위300℃,주온위35℃보지5 min,재이25℃·min-1승지260℃。결과：정기완、병동、을산을지여갑순상린봉간적분리도급검측령민도균부합요구。결론：해법간편、쾌속、령민,결과준학가고,가용우자삼순중잔류유궤용제적측정。
Objective:To establish a capillary GC method for the determination of residual organic solvents in paclitaxel. Meth-ods:The standard solution and test solution was respectively injected into an HP-INNOWAX sillica capillary column by headspace in-jection. The chromatograms were recorded with an FID. The injection port and detector temperature was 250℃ and 300℃, respective-ly, the initial column temperature was kept at 35℃ for 5 min, and then was risen to 260℃ at the rate of 25℃/min. Results:The four residual organic solvents could be separated completely. The resolution and sensitivity were acceptable. Conclusion: The method is proved to be simple, rapid, sensitive and reliable, and can be used in the determination of residual organic solvents in paclitaxel.
