CAJ | 학술논문

目的：观察七叶皂苷钠对沙土鼠脑缺血再灌注模型脑组织匀浆中内皮素( ET)和降钙素基因相关肽( CGRP)含量的影响。方法：采用结扎双侧颈总动脉缺血10 min再灌注2 h,建立沙土鼠脑缺血再灌注模型。七叶皂苷钠(10,20,40 mg·kg-1)术前3 d开始腹腔注射给药,qd,术后1 h给药1次。再灌注2 h后,放射免疫法测定脑组织ET和CGRP含量。结果：七叶皂苷钠各剂量组可降低脑组织ET水平至28.69~37.03 ng·L-1,与模型组比较有显著性差异(P<0.05或0.01),对脑组织CGRP水平则无明显影响(P>0.05)。结论：七叶皂苷钠可能通过降低脑组织ET水平,实现对沙土鼠脑缺血再灌注损伤的保护作用。
목적：관찰칠협조감납대사토서뇌결혈재관주모형뇌조직균장중내피소( ET)화강개소기인상관태( CGRP)함량적영향。방법：채용결찰쌍측경총동맥결혈10 min재관주2 h,건립사토서뇌결혈재관주모형。칠협조감납(10,20,40 mg·kg-1)술전3 d개시복강주사급약,qd,술후1 h급약1차。재관주2 h후,방사면역법측정뇌조직ET화CGRP함량。결과：칠협조감납각제량조가강저뇌조직ET수평지28.69~37.03 ng·L-1,여모형조비교유현저성차이(P<0.05혹0.01),대뇌조직CGRP수평칙무명현영향(P>0.05)。결론：칠협조감납가능통과강저뇌조직ET수평,실현대사토서뇌결혈재관주손상적보호작용。
Objective:To investigate the effects of sodium aescinate on endothelin ( ET ) and calcitonin gene -related peptide ( CGRP) contents in the gerbils with cerebral ischemia/reperfusion injury ( CI/R) . Methods:The gerbil model of CI/R was prepared by bilateral common carotid arteries ligation for 10 min followed by 2-hour reperfusion. Sodium aescinate (i. p. , 10, 20 and 40 mg· kg-1 ) was administered once a day for 3 days before the operation and once every 1 h after the operation, respectively. The levels of ET and CGRP in brain tissue homogenate were determined by a radioimmunoassay method. Results:Sodium aescinate significantly in-hibited the level of ET (28. 69-37. 03 ng·L-1) in the brain tissue of gerbils with CI/R compared with the model group (P<0. 05 or P<0. 01), while showed no evident influence on CGRP (P>0. 05). Conclusion:Sodium aescinate has obvious protective effects a-gainst CI/R in gerbils, which may be due to its inhibitory action on ET levels in brain tissue.