CAJ | 학술논문

目的：研究下呼吸道感染患儿人类博卡病毒（HBoV）载量与疾病严重程度的相关性。方法对2009年1月至2012年9月间554例因下呼吸道感染住院的5岁以下患儿及195名健康对照组儿童下呼吸道吸出物（NPAs）、鼻咽拭子采用荧光聚合酶链反应（PCR）检测HBoV种类，并同时测定阳性标本感染HBoV的载量，利用商品化试剂盒检测其他种类的呼吸道病毒，结合患儿的临床症状和生化指标进行综合分析。结果实验组与健康对照组HBoV阳性率有明显差异，实验组病毒载量（5．10×106拷贝/mL）明显高于对照组（2．70×103拷贝/mL， P＜0．05）。实验组中混合感染组疾病严重程度为一般和中等的患儿 HBoV 载量（分别为2．70×105、3．85×106拷贝/mL）明显低于严重患儿（8．70×106拷贝/mL，P＜0．05）。在1例新生儿重症肺炎患儿NPAs、血液和粪便中均检测到HBoV，并排除了其他常见呼吸道病毒和细菌感染。结论 HBoV是呼吸道疾病致病病原，混合感染中HBoV载量与疾病严重程度呈正相关，HBoV在某些患儿中可以导致重症肺炎。
목적：연구하호흡도감염환인인류박잡병독（HBoV）재량여질병엄중정도적상관성。방법대2009년1월지2012년9월간554례인하호흡도감염주원적5세이하환인급195명건강대조조인동하호흡도흡출물（NPAs）、비인식자채용형광취합매련반응（PCR）검측HBoV충류，병동시측정양성표본감염HBoV적재량，이용상품화시제합검측기타충류적호흡도병독，결합환인적림상증상화생화지표진행종합분석。결과실험조여건강대조조HBoV양성솔유명현차이，실험조병독재량（5．10×106고패/mL）명현고우대조조（2．70×103고패/mL， P＜0．05）。실험조중혼합감염조질병엄중정도위일반화중등적환인 HBoV 재량（분별위2．70×105、3．85×106고패/mL）명현저우엄중환인（8．70×106고패/mL，P＜0．05）。재1례신생인중증폐염환인NPAs、혈액화분편중균검측도HBoV，병배제료기타상견호흡도병독화세균감염。결론 HBoV시호흡도질병치병병원，혼합감염중HBoV재량여질병엄중정도정정상관，HBoV재모사환인중가이도치중증폐염。
Objective To study the correlation of human bocavirus (HBoV)viral load and disease severity in children with lower respiratory tract infections.Methods Nasopharyngeal aspirates (NPAs ) from 554 in-hospital children with lower respiratory tract infections under 5-year-old and swabs from 1 95 healthy children were collected from January 2009 to September 201 2.The types of HBoV and HBoV viral load were determined by fluorescence polymerase chain reaction (PCR).The other respiratory viruses were determined by commercial kits.The results were analyzed with clinical symptoms and biochemistry parameters.Results The positive rates of HBoV between in-hospital children and healthy children had obvious difference.The HBoV viral loads of in-hospital children (5.1 0 ×1 06 copies/mL)were significantly higher than those of healthy children (2.70 ×1 03 copies/mL,P<0.05 ).Among co-infection in-hospital children,the HBoV viral loads of mild and moderate groups (2.70 ×1 05 and 3.85 ×1 06 copies/mL)were lower than that in severe group (8.70 ×1 06 copies/mL,P<0.05 ).There was 1 case of newborn infant with severe pneumonia, and HBoV were found in NPAs,blood and stool samples without other common respiratory trract virus and bacterium infections.Conclusions HBoV is a pathogen for respiratory tract disease in children.HBoV viral load is correlated positively with disease severity in co-infection patients.HBoV can cause severe pneumonia in some children.