CAJ | 학술논문

目的：采用Meta分析的方法探讨丙型肝炎病毒（HCV）感染者体内抗F蛋白循环抗体的产生。方法检索1968年至2012年11月中国知网、万方数据库、万方医学网、维普、SinoMED、Medline、Pubmed、Splinger-link、Cochrane、Science direct的相关文献，手工检索查全补充，严格根据纳入和排除标准筛选文献，将最终纳入文献采用Revman 5．0软件进行数据合并与分析。采用OQAQ量表对结果进行评价。结果 HCV感染者与健康人群对照，共纳入14项病例对照研究，合计1348例 HCV 感染者，F 蛋白抗体检出率差异有统计学意义（P＜0．00001），合并率的优势比＞1，总体比值比（OR）达64．81[95％可信区间（CI）为29．90～140．49]；HCV感染者与非HCV感染者对照，共纳入11项对照研究，合计923例HCV感染者，F蛋白抗体检出率差异有统计学意义（P＜0．00001），HCV感染者F蛋白检出率是非HCV感染者的53．09倍（95％ CI为23．56～119．66）；HCV感染者F蛋白抗体检出率与核心蛋白抗体检出率对照，共纳入8项对照研究，合计770例HCV感染者，差异有统计学意义（P＜0．00001），率差（RD）为-0．42（95％ CI为-0．59～-0．25），核心蛋白抗体检出率比F蛋白抗体高42％。结论 HCV感染者体内抗F蛋白抗体检出率明显高于健康人和非HCV感染者。F蛋白可诱导HCV感染者产生抗F蛋白特异性循环抗体，是HCV产生的特异蛋白，可为实验室诊断HCV感染和监测干扰素联合治疗HCV效果提供理论依据和数据支持。
목적：채용Meta분석적방법탐토병형간염병독（HCV）감염자체내항F단백순배항체적산생。방법검색1968년지2012년11월중국지망、만방수거고、만방의학망、유보、SinoMED、Medline、Pubmed、Splinger-link、Cochrane、Science direct적상관문헌，수공검색사전보충，엄격근거납입화배제표준사선문헌，장최종납입문헌채용Revman 5．0연건진행수거합병여분석。채용OQAQ량표대결과진행평개。결과 HCV감염자여건강인군대조，공납입14항병례대조연구，합계1348례 HCV 감염자，F 단백항체검출솔차이유통계학의의（P＜0．00001），합병솔적우세비＞1，총체비치비（OR）체64．81[95％가신구간（CI）위29．90～140．49]；HCV감염자여비HCV감염자대조，공납입11항대조연구，합계923례HCV감염자，F단백항체검출솔차이유통계학의의（P＜0．00001），HCV감염자F단백검출솔시비HCV감염자적53．09배（95％ CI위23．56～119．66）；HCV감염자F단백항체검출솔여핵심단백항체검출솔대조，공납입8항대조연구，합계770례HCV감염자，차이유통계학의의（P＜0．00001），솔차（RD）위-0．42（95％ CI위-0．59～-0．25），핵심단백항체검출솔비F단백항체고42％。결론 HCV감염자체내항F단백항체검출솔명현고우건강인화비HCV감염자。F단백가유도HCV감염자산생항F단백특이성순배항체，시HCV산생적특이단백，가위실험실진단HCV감염화감측간우소연합치료HCV효과제공이론의거화수거지지。
Objective To investigate the production of anti-F protein antibodies in patients with hepatitis C virus (HCV)infection by Meta analysis.Methods Relative literatures were searched from the CNKI,Wan Fang,Weipu, SinoMED,Medline,Pubmed,Splingerlink,Cochrane and Science direct from 1 968 to November 201 2,and hand searching investigation was as complement.The literatures were selected according to the inclusion and exclusion criteria.The final literatures were consolidated and analyzed by Revman 5 .0 software.The results were evaluated by Oxman-Guyatt Overview quality assessment questionnaire (OQAQ ). Results The results of patients with HCV infection and healthy subjects were compared,and 1 4 studies with 1 348 patients with HCV infection were enrolled.The positive detection rate of anti-F protein antibodies had statistical significance (P<0.000 01 ).The superiority of pooled rate was>1.The total odds ratio (OR)was 64.81 [95% confidence interval (CI)29.90-140.49].The results of patients with HCV infection and non-HCV infection were compared,and 1 1 studies with 923 patients with HCV infection were enrolled.The positive detection rate of anti-F protein antibodies had statistical significance (P<0.000 01 ).The positive detection rate of patients with HCV infection was 53.09 times for that of patients with non-HCV infection (95%CI 23.56-1 1 9.66).By comparing the positive detection rate of anti-F protein antibodies with that of anti-core protein antibodies in patients with HCV infection,and 8 studies with 770 patients with HCV infection were enrolled,and there was statistical significance (P<0.000 01 ).The rate difference (RD)was -0.42 (95% CI -0.59--0.25 ).The positive detection rate of anti-core protein antibodies was 42%,which was higher than that of anti-F protein antibodies. Conclusions The positive detection rate of anti-F protein antibodies in patients with HCV infection is significantly higher than those in healthy subjects and patients with non-HCV infection.F protein can induce HCV infection producing anti-F protein antibodies,which is a specific protein.It provides a theoretical basis and datum support for the diagnosis of HCV infection and monitoring the interferon combination therapy.