中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
15期
7006-7009
,共4页
左鹏%王爱利%王正云%胡琼洁%邵冰%熊维宁%熊盛道
左鵬%王愛利%王正雲%鬍瓊潔%邵冰%熊維寧%熊盛道
좌붕%왕애리%왕정운%호경길%소빙%웅유저%웅성도
假单胞菌,铜绿%乳铁蛋白%肽类%毒力因子类
假單胞菌,銅綠%乳鐵蛋白%肽類%毒力因子類
가단포균,동록%유철단백%태류%독력인자류
Pseudomonas aeruginosa%Lactoferrin%Peptides%Virulence factors
目的：观察乳铁蛋白多肽嵌合体（LFchimera）对铜绿假单胞菌PAO1及PAO-JP2型菌株QS毒力因子的影响。方法以PAO1和PAO-JP2为试验菌株，分为A、B、C、D四组。A组：未作任何干预的铜绿假单胞菌PAO1；B组：LFchimera（1μmol/L）干预的铜绿假单胞菌PAO1；C组：未作任何干预的铜绿假单胞菌PAO-JP2；D组：LFchimera（1μmol/L）干预的铜绿假单胞菌PAO-JP2。分别测定毒力因子绿脓菌素（氯仿萃取法）、弹性蛋白酶（弹性蛋白-刚果红法）、胞外蛋白水解酶（偶氮酪蛋白法）。结果和A组各毒力因子（绿脓菌素、弹性蛋白酶、蛋白水解酶）的活性表达（7.03±0.14、0.149±0.009、0.808±0.038）比较，B组铜绿假单胞菌PAO1菌株的表达（3.07±0.13、0.046±0.004、0.325±0.022）明显下降，差异有统计学意义（P＜0.05）。C组铜绿假单胞菌PAO-JP2菌株各毒力因子（绿脓菌素、弹性蛋白酶、蛋白水解酶）的活性表达（0.76±0.04、0.015±0.003、0.054±0.006）和D组（0.74±0.05、0.014±0.002、0.053±0.005）比较，差异无统计学意义（P＞0.05)。结论 LFchimera可能通过抑制铜绿假单胞菌QS系统减弱其相关毒力因子的活性，从而发挥抗感染作用。
目的：觀察乳鐵蛋白多肽嵌閤體（LFchimera）對銅綠假單胞菌PAO1及PAO-JP2型菌株QS毒力因子的影響。方法以PAO1和PAO-JP2為試驗菌株，分為A、B、C、D四組。A組：未作任何榦預的銅綠假單胞菌PAO1；B組：LFchimera（1μmol/L）榦預的銅綠假單胞菌PAO1；C組：未作任何榦預的銅綠假單胞菌PAO-JP2；D組：LFchimera（1μmol/L）榦預的銅綠假單胞菌PAO-JP2。分彆測定毒力因子綠膿菌素（氯倣萃取法）、彈性蛋白酶（彈性蛋白-剛果紅法）、胞外蛋白水解酶（偶氮酪蛋白法）。結果和A組各毒力因子（綠膿菌素、彈性蛋白酶、蛋白水解酶）的活性錶達（7.03±0.14、0.149±0.009、0.808±0.038）比較，B組銅綠假單胞菌PAO1菌株的錶達（3.07±0.13、0.046±0.004、0.325±0.022）明顯下降，差異有統計學意義（P＜0.05）。C組銅綠假單胞菌PAO-JP2菌株各毒力因子（綠膿菌素、彈性蛋白酶、蛋白水解酶）的活性錶達（0.76±0.04、0.015±0.003、0.054±0.006）和D組（0.74±0.05、0.014±0.002、0.053±0.005）比較，差異無統計學意義（P＞0.05)。結論 LFchimera可能通過抑製銅綠假單胞菌QS繫統減弱其相關毒力因子的活性，從而髮揮抗感染作用。
목적：관찰유철단백다태감합체（LFchimera）대동록가단포균PAO1급PAO-JP2형균주QS독력인자적영향。방법이PAO1화PAO-JP2위시험균주，분위A、B、C、D사조。A조：미작임하간예적동록가단포균PAO1；B조：LFchimera（1μmol/L）간예적동록가단포균PAO1；C조：미작임하간예적동록가단포균PAO-JP2；D조：LFchimera（1μmol/L）간예적동록가단포균PAO-JP2。분별측정독력인자록농균소（록방췌취법）、탄성단백매（탄성단백-강과홍법）、포외단백수해매（우담락단백법）。결과화A조각독력인자（록농균소、탄성단백매、단백수해매）적활성표체（7.03±0.14、0.149±0.009、0.808±0.038）비교，B조동록가단포균PAO1균주적표체（3.07±0.13、0.046±0.004、0.325±0.022）명현하강，차이유통계학의의（P＜0.05）。C조동록가단포균PAO-JP2균주각독력인자（록농균소、탄성단백매、단백수해매）적활성표체（0.76±0.04、0.015±0.003、0.054±0.006）화D조（0.74±0.05、0.014±0.002、0.053±0.005）비교，차이무통계학의의（P＞0.05)。결론 LFchimera가능통과억제동록가단포균QS계통감약기상관독력인자적활성，종이발휘항감염작용。
Objective To observe the effect of lactoferrin-derived peptides chimera(LFchimera)on the virulence factors of Pseudomonas aeruginosa wild strain PAO1 and mutant strain PAO-JP2. Methods The wild type strain PAO1 and mutant strain PAO-JP2 of P.aeruginosa were used as experimental strains. In this study, there were four groups. Group A:the wild type strain of P.aeruginosa-PAO1 without any intervention, Group B:the wild type strain of P.aeruginosa-PAO1 with LFchimera(1μmol/L), Group C: the mutant strain of P.aeruginosa-PAO-JP2 without any intervention, Group D:the mutant strain of P.aeruginosa-PAO-JP2 with LFchimera(1μmol/L). The expression of their virulence factors was determined respectively, included pyocyanin assay (chloroform extraction method), elastase activity(Elastin-Congo Red method)and extracellular proteolytic enzyme activity (Azocasein method). Results Compared with the group A(7.03±0.14,0.149±0.009,0.808±0.038), the virulence factors production(pyocyanin concentration, elastase activity and proteolytic enzyme activity) in group B (3.07± 0.13,0.046±0.004,0.325±0.022)were all reduced, the difference had statistical significance(P<0.05). Pyocyanin concentration, elastase activity and proteolytic enzyme activity had no statistical difference between the group C (0.76±0.04,0.015±0.003,0.054±0.006)and group D(0.74±0.05,0.014±0.002,0.053±0.005) (P>0.05). Conclusion LFchimera may weaken activity of virulence factors associated QS by inhibiting Pseudomonas aeruginosa QS system, which play a role of anti-infection.
