中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
601-605
,共5页
盐酸小檗碱脂质体%制备%硫酸铵梯度法%包封率%高效液相色谱-蒸发光散射检测器%成分含量
鹽痠小檗堿脂質體%製備%硫痠銨梯度法%包封率%高效液相色譜-蒸髮光散射檢測器%成分含量
염산소벽감지질체%제비%류산안제도법%포봉솔%고효액상색보-증발광산사검측기%성분함량
Bererine hydrochloride liposomes%Preparation%Ammonium sulfate gradient method%Encapsulation efficiency%HPLC-ELSD%Component content
目的：制备盐酸小檗碱脂质体,并测定其包封率和脂质体各成分含量。方法：采用硫酸铵梯度法制备盐酸小檗碱脂质体,以超速离心法、微柱法、超滤法对盐酸小檗碱脂质体包封率的测定方法进行研究,以HPLC-ELSD测定脂质体各成分含量。结果：超速离心法能将未包封药物与脂质体很好地分离,最佳超速离心条件：离心速度为60000 r·min-1,离心时间为1 h,离心温度为10℃,脂质浓度为6 mg·ml-1。结论：包封率测定方法具有简单、快速分离等优点。 HPLC-ELSD能够同时测定脂质体各成分含量。
目的：製備鹽痠小檗堿脂質體,併測定其包封率和脂質體各成分含量。方法：採用硫痠銨梯度法製備鹽痠小檗堿脂質體,以超速離心法、微柱法、超濾法對鹽痠小檗堿脂質體包封率的測定方法進行研究,以HPLC-ELSD測定脂質體各成分含量。結果：超速離心法能將未包封藥物與脂質體很好地分離,最佳超速離心條件：離心速度為60000 r·min-1,離心時間為1 h,離心溫度為10℃,脂質濃度為6 mg·ml-1。結論：包封率測定方法具有簡單、快速分離等優點。 HPLC-ELSD能夠同時測定脂質體各成分含量。
목적：제비염산소벽감지질체,병측정기포봉솔화지질체각성분함량。방법：채용류산안제도법제비염산소벽감지질체,이초속리심법、미주법、초려법대염산소벽감지질체포봉솔적측정방법진행연구,이HPLC-ELSD측정지질체각성분함량。결과：초속리심법능장미포봉약물여지질체흔호지분리,최가초속리심조건：리심속도위60000 r·min-1,리심시간위1 h,리심온도위10℃,지질농도위6 mg·ml-1。결론：포봉솔측정방법구유간단、쾌속분리등우점。 HPLC-ELSD능구동시측정지질체각성분함량。
Objective:To prepare bererine hydrochloride ( BER) liposomes and establish an effective method for the determination of content and entrapment efficiency. Methods:BER liposomes were prepared by ammonium sulfate gradient method. The encapsula-tion efficiency of BER liposomes was respectively determined by supercentrifugation method, microcolumn gel method and ultrafiltration method, and the content of every component in BER liposomes was detected by HPLC-ELSD. Results:The results showed that super-centrifugation method could precisely separate the free drug from the liposomes. The optimum parameters of supercentrifugation method were the centrifugal speed of 60 000 r·min-1 , the centrifugal time of 1 h, the centrifugal temperature of 10℃ and the lipid concentra-tion of 6 mg·ml-1 . Conclusion:The method is simple and sensitive with good separation efficiency. HPLC-ELSD can be used to de-termine the content of every component in BER liposomes.
