CAJ | 학술논문

背景与目的：簇集素蛋白(clusterin，CLU)在肺癌的发生、发展中发挥重要作用。本研究用分泌型CLU体外处理肺癌细胞株，旨在探讨分泌型CLU对肺癌细胞株的影响。方法：用真核表达纯化的分泌型CLU处理非小细胞肺癌细胞株H460、A549，采用Boyden小室试验检测细胞的迁移能力，用CCK8检测细胞的增殖能力，利用芯片技术分析CLU处理H460细胞的microRNA表达谱，然后采用实时荧光定量PCR检测分泌型CLU处理H460细胞中microRNA的表达，初步分析分泌型CLU执行肿瘤生物学功能的microRNA相关分子机制。结果：分泌型CLU处理组H460迁移的细胞是BSA处理组的3.10倍(P<0.0001)；分泌型CLU处理组A549迁移的细胞是BSA处理组的3.40倍(P<0.0001)；分泌型CLU抑制了肺癌细胞株H460/A549的生长(P<0.0001)；利用芯片技术发现microRNA-302b-3p、microRNA-23a-5p和microRNA-101-5p高表达。结论：在非小细胞肺癌细胞株H460/A549中，真核表达的分泌型CLU促进了细胞迁移、抑制了细胞生长，并且能引起microRNA表达谱的改变，本研究为探索分泌型CLU在非小细胞肺癌中的作用提供了重要的实验依据。
배경여목적：족집소단백(clusterin，CLU)재폐암적발생、발전중발휘중요작용。본연구용분비형CLU체외처리폐암세포주，지재탐토분비형CLU대폐암세포주적영향。방법：용진핵표체순화적분비형CLU처리비소세포폐암세포주H460、A549，채용Boyden소실시험검측세포적천이능력，용CCK8검측세포적증식능력，이용심편기술분석CLU처리H460세포적microRNA표체보，연후채용실시형광정량PCR검측분비형CLU처리H460세포중microRNA적표체，초보분석분비형CLU집행종류생물학공능적microRNA상관분자궤제。결과：분비형CLU처리조H460천이적세포시BSA처리조적3.10배(P<0.0001)；분비형CLU처리조A549천이적세포시BSA처리조적3.40배(P<0.0001)；분비형CLU억제료폐암세포주H460/A549적생장(P<0.0001)；이용심편기술발현microRNA-302b-3p、microRNA-23a-5p화microRNA-101-5p고표체。결론：재비소세포폐암세포주H460/A549중，진핵표체적분비형CLU촉진료세포천이、억제료세포생장，병차능인기microRNA표체보적개변，본연구위탐색분비형CLU재비소세포폐암중적작용제공료중요적실험의거。
Background and purpose: Primary study showed that clusterin was associated with tumorigenicity. The goal of the study is to investigate the role of secretory clusterin in non-small cell lung cancer cell A549/H460. Methods: The lung cancer cell A549/H460 was treated with purified secretory clusterin, the Boyden Chamber migration assay was used to detected the migration of the lung cancer cell;the CCK8 assay was used to detected the growth of the cells;microRNA expression spectrum in H460 treated with secretory clusterin was analyzed, after that, we used the real-time florescence quantification detected the expression of microRNA in H460, and the biological function of microRNA molecular mechanisms of secretory clusterin was analyzed. Results: Secretory clusterin promoted the migration in A549/H460 (P<0.000 1);Secretory clusterin inhibited the growth in H460/A549 (P<0.000 1);MicroRNA-302b-3p, microRNA-23a-5p and microRNA-101-5p was overexpressed in H460 when treated with secretory clusterin. Conclusion:Secretory clusterin could promote the migration and inhibit the growth of lung cancer cell;It could change the microRNA expression spectrum as well. Our studies revealed that secretory clusterin could be used as a tool for further study, and it is a potential target in the treatment of lung cancer.