CAJ | 학술논문

背景：骨髓间充质干细胞向神经细胞诱导分化可为神经系统受损伤后的修复和再生带来了新的希望。 　　目的：探讨骨髓间充质干细胞在视网膜干细胞培养上清液诱导条件下向神经元细胞分化。 　　方法：采用全骨髓培养方法，用视网膜干细胞培养上清液诱导骨髓间充质干细胞，通过免疫荧光染色鉴定其分化的结果。 　　结果与结论：诱导72 h，骨髓间充质干细胞表达神经干细胞的特异性抗体巢蛋白和神经元中的标志性微管相关蛋白微管相关蛋白2。视网膜干细胞培养上清液能够促进骨髓间充质干细胞向神经元样细胞分化，提示视网膜干细胞可能分泌神经生长因子。
배경：골수간충질간세포향신경세포유도분화가위신경계통수손상후적수복화재생대래료신적희망。 　　목적：탐토골수간충질간세포재시망막간세포배양상청액유도조건하향신경원세포분화。 　　방법：채용전골수배양방법，용시망막간세포배양상청액유도골수간충질간세포，통과면역형광염색감정기분화적결과。 　　결과여결론：유도72 h，골수간충질간세포표체신경간세포적특이성항체소단백화신경원중적표지성미관상관단백미관상관단백2。시망막간세포배양상청액능구촉진골수간충질간세포향신경원양세포분화，제시시망막간세포가능분비신경생장인자。
BACKGROUND:Differentiation of bone marrow mesenchymal stem cells into nerve cells has brought a new hope for the repair and regeneration after nervous system injuries. OBJECTIVE:To study the neuron-like differentiation of bone marrow mesenchymal stem cells induced by the retinal stem cellculture supernatant. METHODS:The whole bone marrow culture was applied. The differentiation results of bone marrow mesenchymal stem cells induced by retinal stem cellculture supernatants were identified by immunofluorescence method. RESULTS AND CONCLUSION:After 72 hours of induction, bone marrow mesenchymal stem cells express nestin and microtubule-associated protein 2. Retinal stem cellculture supernatant can promote bone marrow mesenchymal stem cells differentiating into neuron-like cells, suggesting that retinal stem cells may secrete nerve growth factor.