中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
41期
7335-7340
,共6页
组织构建%组织构建综述%周围神经%神经损伤%干细胞%许旺细胞%间充质干细胞%脂肪干细胞%神经干细胞%胚胎干细胞%支架%生长因子
組織構建%組織構建綜述%週圍神經%神經損傷%榦細胞%許旺細胞%間充質榦細胞%脂肪榦細胞%神經榦細胞%胚胎榦細胞%支架%生長因子
조직구건%조직구건종술%주위신경%신경손상%간세포%허왕세포%간충질간세포%지방간세포%신경간세포%배태간세포%지가%생장인자
peripheral nerves%nerve injuries%stem cells%Schwann cells%biocompatible materials
背景：近年来，随着生物工程技术以及组织工程化神经的发展给周围神经缺损的治疗带来了新的希望，已逐渐成为研究的焦点。目的：从种子细胞、生物材料以及构建周围神经组织技术3个方面综述组织工程方法修复周围神经损伤的新进展。方法：由第一作者在2013年7月应用计算机检索PubMed 数据库及CNKI 数据库，英文关键词为“tissue engineering，peripheral nerves，nerve injuries，stem cel s，schwann cel s，scaffold，growth factor”，中文关键词为“组织工程，周围神经，神经损伤，干细胞，许旺细胞，支架，生长因子”。选择内容与神经组织工程、周围神经损伤修复相关的文章，同一领域文献则选择近期发表或发表在权威杂志文章，共纳入63篇文献。结果与结论：现阶段组织工程方法修复周围神经损伤的研究虽已取得很大进展，但大多停留于实验探索阶段。将组织工程神经应用于临床尚存下列问题亟待解决：①种子细胞来源及伦理。②细胞扩增后移植的免疫排斥。③移植细胞稳定性问题及致瘤性。④神经支架材料的降解速度、最佳孔隙率、导管厚度、形状等。⑤体外神经构建后移植修复时机。⑥各种神经生物因子的局部释放与调控等等。随着科技的发展，期待上述问题的解决，从而使得众多临床神经损伤患者受益。
揹景：近年來，隨著生物工程技術以及組織工程化神經的髮展給週圍神經缺損的治療帶來瞭新的希望，已逐漸成為研究的焦點。目的：從種子細胞、生物材料以及構建週圍神經組織技術3箇方麵綜述組織工程方法脩複週圍神經損傷的新進展。方法：由第一作者在2013年7月應用計算機檢索PubMed 數據庫及CNKI 數據庫，英文關鍵詞為“tissue engineering，peripheral nerves，nerve injuries，stem cel s，schwann cel s，scaffold，growth factor”，中文關鍵詞為“組織工程，週圍神經，神經損傷，榦細胞，許旺細胞，支架，生長因子”。選擇內容與神經組織工程、週圍神經損傷脩複相關的文章，同一領域文獻則選擇近期髮錶或髮錶在權威雜誌文章，共納入63篇文獻。結果與結論：現階段組織工程方法脩複週圍神經損傷的研究雖已取得很大進展，但大多停留于實驗探索階段。將組織工程神經應用于臨床尚存下列問題亟待解決：①種子細胞來源及倫理。②細胞擴增後移植的免疫排斥。③移植細胞穩定性問題及緻瘤性。④神經支架材料的降解速度、最佳孔隙率、導管厚度、形狀等。⑤體外神經構建後移植脩複時機。⑥各種神經生物因子的跼部釋放與調控等等。隨著科技的髮展，期待上述問題的解決，從而使得衆多臨床神經損傷患者受益。
배경：근년래，수착생물공정기술이급조직공정화신경적발전급주위신경결손적치료대래료신적희망，이축점성위연구적초점。목적：종충자세포、생물재료이급구건주위신경조직기술3개방면종술조직공정방법수복주위신경손상적신진전。방법：유제일작자재2013년7월응용계산궤검색PubMed 수거고급CNKI 수거고，영문관건사위“tissue engineering，peripheral nerves，nerve injuries，stem cel s，schwann cel s，scaffold，growth factor”，중문관건사위“조직공정，주위신경，신경손상，간세포，허왕세포，지가，생장인자”。선택내용여신경조직공정、주위신경손상수복상관적문장，동일영역문헌칙선택근기발표혹발표재권위잡지문장，공납입63편문헌。결과여결론：현계단조직공정방법수복주위신경손상적연구수이취득흔대진전，단대다정류우실험탐색계단。장조직공정신경응용우림상상존하렬문제극대해결：①충자세포래원급윤리。②세포확증후이식적면역배척。③이식세포은정성문제급치류성。④신경지가재료적강해속도、최가공극솔、도관후도、형상등。⑤체외신경구건후이식수복시궤。⑥각충신경생물인자적국부석방여조공등등。수착과기적발전，기대상술문제적해결，종이사득음다림상신경손상환자수익。
BACKGROUND:Recent development of bioengineering technology and tissue-engineered nerve brings a new hope for the treatment of peripheral nerve injuries, which has gradual y become a research spot. OBJECTIVE:To review the new progress in the repair of peripheral nerve injuries using seed cells, biomaterials and tissue-engineered nerve construction technology. METHODS:PubMed and CNKI were searched by the first authors for articles concerning nerve tissue engineering and repair of peripheral nerve injuries published prior to July 2013. The keywords were“tissue engineering, peripheral nerves, nerve injuries, stem cells, Schwann cells, scaffold, growth factor”in English and Chinese, respectively. The articles published recently or in the authorized journals were preferred in the same field. Final y, 63 articles were included in result analysis. RESULTS AND CONCLUSION:Up to now, there is a great advance in the tissue engineering technology for the repair of peripheral nerve injuries. However, most studies are stil in experimental step. For the clinical application of nerve tissue engineering, some problems to be solved include:(1) source and ethics of seed cells;(2) immunological rejection fol owing cellproliferation and transplantation;(3) stability and oncogenicity of transplanted cells;(4) degradation rate, optimal porosity, tube thickness and shape;(5) repair timing for in vitro tissue-engineered nerve construction;(6) local release and regulation of various neurobiological factors. With the development of science, many patients with nerve injuries can profit from the solve of these problems.
