中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2012年
1期
37-41
,共5页
申斯思%郑晓聪%刘荭%史秀杰%贾鹏%兰文升%何俊强%张朋%余剑敏%杜娟%肖启明%康林
申斯思%鄭曉聰%劉葒%史秀傑%賈鵬%蘭文升%何俊彊%張朋%餘劍敏%杜娟%肖啟明%康林
신사사%정효총%류홍%사수걸%가붕%란문승%하준강%장붕%여검민%두연%초계명%강림
传染性造血器官坏死病病毒%糖蛋白%同源性%进化分析
傳染性造血器官壞死病病毒%糖蛋白%同源性%進化分析
전염성조혈기관배사병병독%당단백%동원성%진화분석
infectious hematopoietic necrosis virus%glycoprotein%homology%phylogenetic analysis
对所分离编号为20101008和20101032的两株传染性造血器官坏死病病毒（IHNV）的糖蛋白编码基因进行遗传进化分析,以揭示我国毒株与其他不同国家和地区毒株间的遗传进化关系。以反转录RCR（RT-PCR）法扩增其糖蛋白（G）编码基因,然后克隆入PMD-18T载体并测序。应用DNAStar和MEGA4.0软件,将20101008和20101032的G基因与多株GenBank中已发表的IHNV毒株相应基因进行比较。结果表明两株病毒间G编码基因的核苷酸和推导出来的氨基酸同源性分别为98%和81.9%,其氨基酸序列分别发生了35和20处氨基酸的替换。20101008和20101032与日本、韩国分离株同源性较高,核苷酸及推导出来的氨基酸同源性分别为93.3%～96.4%和75.9%～84.6%;两株病毒在进化关系上亲缘关系最近,属于同一分支,均为基因U型传染性造血器官坏死病病毒,但其G基因的核苷酸序列以及推导出来的氨基酸序列与已知的基因U型IHNV都有一定的差异。
對所分離編號為20101008和20101032的兩株傳染性造血器官壞死病病毒（IHNV）的糖蛋白編碼基因進行遺傳進化分析,以揭示我國毒株與其他不同國傢和地區毒株間的遺傳進化關繫。以反轉錄RCR（RT-PCR）法擴增其糖蛋白（G）編碼基因,然後剋隆入PMD-18T載體併測序。應用DNAStar和MEGA4.0軟件,將20101008和20101032的G基因與多株GenBank中已髮錶的IHNV毒株相應基因進行比較。結果錶明兩株病毒間G編碼基因的覈苷痠和推導齣來的氨基痠同源性分彆為98%和81.9%,其氨基痠序列分彆髮生瞭35和20處氨基痠的替換。20101008和20101032與日本、韓國分離株同源性較高,覈苷痠及推導齣來的氨基痠同源性分彆為93.3%～96.4%和75.9%～84.6%;兩株病毒在進化關繫上親緣關繫最近,屬于同一分支,均為基因U型傳染性造血器官壞死病病毒,但其G基因的覈苷痠序列以及推導齣來的氨基痠序列與已知的基因U型IHNV都有一定的差異。
대소분리편호위20101008화20101032적량주전염성조혈기관배사병병독（IHNV）적당단백편마기인진행유전진화분석,이게시아국독주여기타불동국가화지구독주간적유전진화관계。이반전록RCR（RT-PCR）법확증기당단백（G）편마기인,연후극륭입PMD-18T재체병측서。응용DNAStar화MEGA4.0연건,장20101008화20101032적G기인여다주GenBank중이발표적IHNV독주상응기인진행비교。결과표명량주병독간G편마기인적핵감산화추도출래적안기산동원성분별위98%화81.9%,기안기산서렬분별발생료35화20처안기산적체환。20101008화20101032여일본、한국분리주동원성교고,핵감산급추도출래적안기산동원성분별위93.3%～96.4%화75.9%～84.6%;량주병독재진화관계상친연관계최근,속우동일분지,균위기인U형전염성조혈기관배사병병독,단기G기인적핵감산서렬이급추도출래적안기산서렬여이지적기인U형IHNV도유일정적차이。
The genetic evolution analysis was conducted for the glycoprotein genes in two isolated strains designated as 20101008 and 20101032 respectively of infectious hematopoietic necrosis virus（IHNV）in order to reveal the phylogenetic relationships of our strains and other strains in different countries and regions.The glycoprotein genes were amplified by RT-PCR and sequenced after being cloned directly into the pMD18-T.The sequences of G genes of 20101008 and 20101032 were compared with the sequences of other IHNV strains in GenBank by DNAStar and MEGA4.0 software.The results showed that the nucleotide and the deduced amino acid identities on G gene of our two strains were 98% and 81.9% respectively.Further analysis of the deduced amino acid sequences indicated 35 and 20 of amino acid residues of G gene were substituted in 20101008 and 20101032.They shared closer identity with strains isolated from Japan and South Korea,and the rate of nucleotide and amino acid homology of G gene were 93.3%～96.4% and 75.9%～84.6% respectively.The two strains 20101008 and 20101032 had the closest genetic relatives and belonged to the same branch and they belonged to genotype U.However,the G gene diverges from known strains at either nucleotide level or amino acid level.
