CAJ | 학술논문

针对非洲猪瘟病毒（ASFV）P54基因建立荧光PCR检测方法,通过优化引物、TaqMan探针浓度及反应温度,研发了ASFV检测的荧光PCR检测试剂盒。经灵敏度的重复性试验及3种不同型号荧光PCR仪使用验证,所研发的试剂盒灵敏度为101copy/μL,适用于不同型号荧光PCR仪使用,可用于对非洲猪瘟病毒的快速检测。
침대비주저온병독（ASFV）P54기인건립형광PCR검측방법,통과우화인물、TaqMan탐침농도급반응온도,연발료ASFV검측적형광PCR검측시제합。경령민도적중복성시험급3충불동형호형광PCR의사용험증,소연발적시제합령민도위101copy/μL,괄용우불동형호형광PCR의사용,가용우대비주저온병독적쾌속검측。
A fluorescence polymerase chain reaction（PCR）assay was developed with ASFV p54 gene for the detection of African swine fever virus.And the ASFV fluorescent quantitative polymerase chain reaction（PCR）test kit was obtained through optimization of the primer and the fluorescence labeled TaqMan probe concentration and reaction temperature.Several snsitivity tests and verification with three different instrument models showed that,the sensitivity of kit was 101 copy/μL and suitable for different types of instrument.All these proved that the kit could be used for fast detection of ASFV.