中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
15期
7018-7021
,共4页
易善永%南克俊%阮静%张丽娟%柯洋
易善永%南剋俊%阮靜%張麗娟%柯洋
역선영%남극준%원정%장려연%가양
肝肿瘤%肿瘤干细胞%内皮,血管%血管内皮细胞
肝腫瘤%腫瘤榦細胞%內皮,血管%血管內皮細胞
간종류%종류간세포%내피,혈관%혈관내피세포
Liver neoplasms%Neoplastic stem cells%Endothelium,vascular%Human umbilical vein endothelial cells
目的：探讨血管内皮细胞对肝癌干细胞样细胞增殖及成瘤的影响，并初步了解血管微环境对肝癌干细胞样细胞作用。方法用磁珠分选技术分选MHCC97H细胞株中的肝癌干细胞样细胞，将其分别接种到普通软琼脂和内皮细胞条件软琼脂中，观察集落形成情况；将其与血管内皮细胞混合细胞接种到裸鼠皮下，观察移植瘤形成情况。结果肝癌干细胞样细胞在普通培养基和内皮细胞条件培养基中集落形成率分别为：（14.25±2.94）% vs.（33.49±5.46）%，差异具有统计学意义（P＝0.036）。两组移植瘤的体积和重量分别为（1442.73±67.51）mm3 vs.（862.93±135.12）mm3；（1.45±0.15）g vs.（0.91±0.19）g，差异具有统计学意义（P＝0.008；P＝0.043）。结论血管内皮细胞是肿瘤干细胞血管微环境的重要组成部分，血管内皮细胞不仅在体外培养时能够促进肝癌干细胞样细胞的生长与增殖，而且在体内也能促进其移植瘤的生长。
目的：探討血管內皮細胞對肝癌榦細胞樣細胞增殖及成瘤的影響，併初步瞭解血管微環境對肝癌榦細胞樣細胞作用。方法用磁珠分選技術分選MHCC97H細胞株中的肝癌榦細胞樣細胞，將其分彆接種到普通軟瓊脂和內皮細胞條件軟瓊脂中，觀察集落形成情況；將其與血管內皮細胞混閤細胞接種到裸鼠皮下，觀察移植瘤形成情況。結果肝癌榦細胞樣細胞在普通培養基和內皮細胞條件培養基中集落形成率分彆為：（14.25±2.94）% vs.（33.49±5.46）%，差異具有統計學意義（P＝0.036）。兩組移植瘤的體積和重量分彆為（1442.73±67.51）mm3 vs.（862.93±135.12）mm3；（1.45±0.15）g vs.（0.91±0.19）g，差異具有統計學意義（P＝0.008；P＝0.043）。結論血管內皮細胞是腫瘤榦細胞血管微環境的重要組成部分，血管內皮細胞不僅在體外培養時能夠促進肝癌榦細胞樣細胞的生長與增殖，而且在體內也能促進其移植瘤的生長。
목적：탐토혈관내피세포대간암간세포양세포증식급성류적영향，병초보료해혈관미배경대간암간세포양세포작용。방법용자주분선기술분선MHCC97H세포주중적간암간세포양세포，장기분별접충도보통연경지화내피세포조건연경지중，관찰집락형성정황；장기여혈관내피세포혼합세포접충도라서피하，관찰이식류형성정황。결과간암간세포양세포재보통배양기화내피세포조건배양기중집락형성솔분별위：（14.25±2.94）% vs.（33.49±5.46）%，차이구유통계학의의（P＝0.036）。량조이식류적체적화중량분별위（1442.73±67.51）mm3 vs.（862.93±135.12）mm3；（1.45±0.15）g vs.（0.91±0.19）g，차이구유통계학의의（P＝0.008；P＝0.043）。결론혈관내피세포시종류간세포혈관미배경적중요조성부분，혈관내피세포불부재체외배양시능구촉진간암간세포양세포적생장여증식，이차재체내야능촉진기이식류적생장。
Objective To investigate the effects of vascular endothelial cells(VECs) on the proliferation in vitro and tumorigenesis in vivo of liver cancer stem-like cells(LCSLC). Methods LCSLC were inoculated in both normal serum-containing media and human umbilical vein endothelial cells (HUVECs) conditioned serum-containing media. After 2 weeks, the number of the colony formation was assessed by counting under microscope. LCSLC either alone or in combination with HUVECs were inoculated into the bilateral back of every nude mouse. After 4 weeks, xenograft tumors were measured. Results The ratios of colony formation of LCSLC in HUVEC conditioned media and unconditioned identical media were (33.49±5.46)% and (14.25±2.94)%, which demonstrated statistical significance(P=0.036). The results strongly indicated that LCSLC cultures grown in HUVEC conditioned media formed significantly more colony compared with LCSLC grown in unconditioned identical media. The volume and weight of xenograft tumors of LCSLC group and mixed cells group were (1442.73±67.51)mm3 vs. (862.93±135.12)mm3;(1.45±0.15)g vs. (0.91±0.19)g. There were significant differences between the two groups (P=0.008 or P=0.043). The data suggested that HUVEC could promote the growth of xenograft tumors in vivo, also. Conclusion VECs are critical component of the cancer stem cells vascular niche. VECs could enhance the self-renewal and proliferation capacity of LCSLC in vitro, and they also accelerated the initiation and growth of xenograft tumors.
