CAJ | 학술논문

目的：观察骨髓间充质干细胞（BMSCs）移植对大鼠脑出血后神经可塑性的影响。方法利用脑立体定位仪制作大鼠脑出血模型；将120只大鼠随机分成BMSCs移植组、模型组，每组各60只；利用透射电镜观察血肿周围神经组织超微结构及神经突触的变化并应用Simple-PCI图像分析系统，选定测量参数计算突触数量，突触界面曲率，突触后致密区宽度及突触间隙；利用免疫组化技术检测大鼠血肿周围神经组织中神经突触相关蛋白Shank1、Nestin的表达；利用Berderson评分标准对大鼠进行神经功能评价。结果透射电镜观察，BMSCs移植组脑出血灶周围可见大量新生神经元及神经胶质细胞，神经突触数量为16.27±2.14，神经突触界面曲率为1.57±0.04，突触后致密区为68.32±10.54，突触间隙为14.65±1.58，与模型组比较差异有统计学意义（P<0.01）。BMSCs移植组及模型组Shank1表达分别为：75.82±10.65、14.33±1.14，两者比较差异有统计学意义（P<0.01）；BMSCs移植组及模型组Nestin阳性细胞表达分别为：68.87±7.46、12.64±0.07，两者比较差异有统计学意义（P<0.01）。BMSCs移植组神经功能评分明显降低，与模型组比较有统计学意义（P<0.05）。结论 BMSCs 可促进大鼠脑出血后神经组织修复，增强神经可塑性，促进神经功能恢复。
목적：관찰골수간충질간세포（BMSCs）이식대대서뇌출혈후신경가소성적영향。방법이용뇌입체정위의제작대서뇌출혈모형；장120지대서수궤분성BMSCs이식조、모형조，매조각60지；이용투사전경관찰혈종주위신경조직초미결구급신경돌촉적변화병응용Simple-PCI도상분석계통，선정측량삼수계산돌촉수량，돌촉계면곡솔，돌촉후치밀구관도급돌촉간극；이용면역조화기술검측대서혈종주위신경조직중신경돌촉상관단백Shank1、Nestin적표체；이용Berderson평분표준대대서진행신경공능평개。결과투사전경관찰，BMSCs이식조뇌출혈조주위가견대량신생신경원급신경효질세포，신경돌촉수량위16.27±2.14，신경돌촉계면곡솔위1.57±0.04，돌촉후치밀구위68.32±10.54，돌촉간극위14.65±1.58，여모형조비교차이유통계학의의（P<0.01）。BMSCs이식조급모형조Shank1표체분별위：75.82±10.65、14.33±1.14，량자비교차이유통계학의의（P<0.01）；BMSCs이식조급모형조Nestin양성세포표체분별위：68.87±7.46、12.64±0.07，량자비교차이유통계학의의（P<0.01）。BMSCs이식조신경공능평분명현강저，여모형조비교유통계학의의（P<0.05）。결론 BMSCs 가촉진대서뇌출혈후신경조직수복，증강신경가소성，촉진신경공능회복。
Objective To observe the influence of neuroplasticity of hemorrhage in rats after transplantation of marrow mesenchymal stem cell. Methods The models were made by brain solid positioner. Rats were randomly divided into BMSCs group and control group.Observe the ultrastructure of nervous tissue and the change of neurosynaptic near hematoma by transmission electron microscope. Study the neurosynaptic quantificationally. The expression of Shank1and Nestin proteins which were related with synapse were detected by using immunohistochemistry. Evaluate the neural function of rats. Results Much new neuron and neurogliocyte were observed around cerebral hemorrhage in BMSCs group by transmission electron microscope. The number of neurosynaptic increased obviously. The curvature of synaptic boundary magnified and dense area of postsynatic thicken. The gap of cynapse narrowed obviously. Compared with control group, it was statistically significant (P<0.01). The expression of Shank1 protein around cerebral hemorrhage increased in BMSCs group. Compared with control group, it was statistically significant. The expression of Nestin positive cells around cerebral hemorrhage were detected in BMSCs group. There is no Nestin positive cells in control group. The neurological scores of BMSCs group was significantly decreased. Compared with control group, it was statistically significant. Conclusion The BMSCs transplantation can repair the nervous tissue,strengthen the plasticity of nerve and promote the recovery of neural function.