现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2014年
5期
998-1001
,共4页
黄连素%肺癌%增殖%上皮-间质转化(EMT)%迁移%侵袭
黃連素%肺癌%增殖%上皮-間質轉化(EMT)%遷移%侵襲
황련소%폐암%증식%상피-간질전화(EMT)%천이%침습
Berberine%lung cancer%Proliferation%epithelial - to - mesenchymal transition(EMT)%migration%inva-sion
目的：研究黄连素（BBR）对 A549细胞增殖、迁移和侵袭的影响，初步探讨 BBR 抑制 A549细胞转移的机制。方法：用不同浓度的 BBR 作用于 A549细胞，MTT 法测定 A549细胞增殖水平，流式细胞仪检测 A549细胞的凋亡，Transwell 实验测定 A549细胞的迁移和侵袭能力，Western blot 测定 A549细胞中与 EMT 相关的蛋白 E - cadherin、Vimentin、Slug、Snail 的表达。结果：BBR 以浓度及时间依赖的方式抑制 A549细胞的增殖；流式细胞仪检测发现 BBR 作用于 A549细胞后凋亡率呈剂量依赖性增加；Western blot 显示 BBR 作用 A549细胞后，E - cadherin 表达升高，Vimentin、Slug、Snail 表达降低，Transwell 实验显示 BBR 作用于 A549细胞后，能够抑制其迁移和侵袭。结论：BBR 能抑制 A549细胞增殖，诱导其凋亡，通过促进 E - cadherin 表达和抑制Vimentin、Slug、Snail 的表达来抑制 EMT 的发生，从而抑制了 A549细胞的迁移和侵袭。
目的：研究黃連素（BBR）對 A549細胞增殖、遷移和侵襲的影響，初步探討 BBR 抑製 A549細胞轉移的機製。方法：用不同濃度的 BBR 作用于 A549細胞，MTT 法測定 A549細胞增殖水平，流式細胞儀檢測 A549細胞的凋亡，Transwell 實驗測定 A549細胞的遷移和侵襲能力，Western blot 測定 A549細胞中與 EMT 相關的蛋白 E - cadherin、Vimentin、Slug、Snail 的錶達。結果：BBR 以濃度及時間依賴的方式抑製 A549細胞的增殖；流式細胞儀檢測髮現 BBR 作用于 A549細胞後凋亡率呈劑量依賴性增加；Western blot 顯示 BBR 作用 A549細胞後，E - cadherin 錶達升高，Vimentin、Slug、Snail 錶達降低，Transwell 實驗顯示 BBR 作用于 A549細胞後，能夠抑製其遷移和侵襲。結論：BBR 能抑製 A549細胞增殖，誘導其凋亡，通過促進 E - cadherin 錶達和抑製Vimentin、Slug、Snail 的錶達來抑製 EMT 的髮生，從而抑製瞭 A549細胞的遷移和侵襲。
목적：연구황련소（BBR）대 A549세포증식、천이화침습적영향，초보탐토 BBR 억제 A549세포전이적궤제。방법：용불동농도적 BBR 작용우 A549세포，MTT 법측정 A549세포증식수평，류식세포의검측 A549세포적조망，Transwell 실험측정 A549세포적천이화침습능력，Western blot 측정 A549세포중여 EMT 상관적단백 E - cadherin、Vimentin、Slug、Snail 적표체。결과：BBR 이농도급시간의뢰적방식억제 A549세포적증식；류식세포의검측발현 BBR 작용우 A549세포후조망솔정제량의뢰성증가；Western blot 현시 BBR 작용 A549세포후，E - cadherin 표체승고，Vimentin、Slug、Snail 표체강저，Transwell 실험현시 BBR 작용우 A549세포후，능구억제기천이화침습。결론：BBR 능억제 A549세포증식，유도기조망，통과촉진 E - cadherin 표체화억제Vimentin、Slug、Snail 적표체래억제 EMT 적발생，종이억제료 A549세포적천이화침습。
Objective:To study the effect of Berberine on Proliferation,migration and invasion of A549 cells. Methods:The Proliferation and aPoPtosis of A549 cells were determined by MTT and FACS resPectively. The rate of A549 cell migration was observed by Transwell assay. The exPression of E - cadherin,Vimentin,Slug,Snail in A549 cells effected by Berberine were assessed by Western blot. Results:The Proliferation of A549 cell could be significant-ly inhibited within limited duration and dosage(P ﹤ 0. 05). Also exPression of Vimentin,Snail and Slug of gene were decreased but E - cadherin increased after Berberine treatment(P ﹤ 0. 05). Conclusion:Berberine can inhibit the mi-gration abilities of A549 cells that might be related to down - regulating the exPression of Vimentin,Snail and uP -regulating the exPression of E - cadherin.
