CAJ | 학술논문

目的：研究降低 IKKα的表达对人胃癌细胞迁移和侵袭能力的影响。方法：利用 Western blot 检测胃癌细胞系中 IKKα蛋白的表达水平，选择 IKKα表达较高的细胞系 MKN28，转染下调 IKKα的慢病毒，建立稳定转染细胞株（标记为 MKN28- LV - shcontrol 和 MKN28- LV - shIKKα），并利用 Western blot、RT - PCR 验证病毒转染效果。采用 Transwell 实验和划痕实验检测低表达 IKKα对胃癌细胞迁移和侵袭能力的影响。结果：Western blot 结果显示胃癌细胞系中 MKN28细胞 IKKα蛋白表达水平相对较高，转染慢病毒干扰载体成功后利用 Western blot 及 RT - PCR 实验证实实验组 MKN28- LV - shIKKα较对照组 MKN28- LV - shcontrol 中IKKα蛋白及 mRNA 水平明显降低。利用 Transwell 及划痕实验证实实验组 MKN28- LV - shIKKα较对照组MKN28- LV - shcontrol 细胞迁移及侵袭能力明显降低。结论：IKKα与胃癌的迁移及侵袭密切相关，降低IKKα的表达水平能显著抑制胃癌细胞的迁移和侵袭能力，提示 IKKα在胃癌的转移中可能发挥着重要作用。
목적：연구강저 IKKα적표체대인위암세포천이화침습능력적영향。방법：이용 Western blot 검측위암세포계중 IKKα단백적표체수평，선택 IKKα표체교고적세포계 MKN28，전염하조 IKKα적만병독，건립은정전염세포주（표기위 MKN28- LV - shcontrol 화 MKN28- LV - shIKKα），병이용 Western blot、RT - PCR 험증병독전염효과。채용 Transwell 실험화화흔실험검측저표체 IKKα대위암세포천이화침습능력적영향。결과：Western blot 결과현시위암세포계중 MKN28세포 IKKα단백표체수평상대교고，전염만병독간우재체성공후이용 Western blot 급 RT - PCR 실험증실실험조 MKN28- LV - shIKKα교대조조 MKN28- LV - shcontrol 중IKKα단백급 mRNA 수평명현강저。이용 Transwell 급화흔실험증실실험조 MKN28- LV - shIKKα교대조조MKN28- LV - shcontrol 세포천이급침습능력명현강저。결론：IKKα여위암적천이급침습밀절상관，강저IKKα적표체수평능현저억제위암세포적천이화침습능력，제시 IKKα재위암적전이중가능발휘착중요작용。
Objective:To exPlore the migration and invasion ability of gastric cancer cells after silencing IKKα ex-Pression. Methods:We used Western blot to detect the IKKα exPression in different gastric cancer cells,and then transfected IKKα shRNA lentivirus and control lentivirus into MKN - 28 cells exPressing high level of IKKα. Western blot and RT - PCR were Performed to verified the silencing effect. And then we conducted transwell migration and in-vasion assay and the wound - healing assay to deliberate the role of IKKα in GC metastasis. Results:Western blot showed that the exPression level of IKKα in MKN28 cells were higher than other gastric cancer cell lines. After trans-fection of IKKα shRNA,the Protein and mRNA level of IKKα in the MKN28 - LV - shIKKα was significantly de-creased. The migration and invasion ability of MKN28 - LV - shIKKα was significantly decreased comPared with MKN28 - LV - shcontrol. Conclusion:Silencing of IKKα significantly decreased the invasion and migration ability of gastric cancer cells. Targeting IKKα may therefore Provide a Promising theraPeutic strategy for gastric cancer Patients.