浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2014年
1期
1-6
,共6页
成晓越%王栩鸣%杨勇%周洁%余初浪%韩雪颖%程晔%严成其%陈剑平
成曉越%王栩鳴%楊勇%週潔%餘初浪%韓雪穎%程曄%嚴成其%陳劍平
성효월%왕허명%양용%주길%여초랑%한설영%정엽%엄성기%진검평
疣粒野生稻%水稻白叶枯病%一氧化氮%亚细胞定位%细胞壁增厚
疣粒野生稻%水稻白葉枯病%一氧化氮%亞細胞定位%細胞壁增厚
우립야생도%수도백협고병%일양화담%아세포정위%세포벽증후
Oryza meyeriana%rice bacterial blight%nitric oxide%subcellular location%cell-wall thickening
疣粒野生稻高抗水稻白叶枯病，但是其具体的抗性机制目前仍不清楚。一氧化氮（ nitric oxide，NO）是一种重要的信号分子，在植物的抗病反应中起到了重要的作用，然而对于NO是否参与疣粒野生稻对水稻白叶枯病的抗性目前仍缺乏研究。以抗病的疣粒野生稻和感病的水稻品种日本晴为材料，研究了接种白叶枯病菌对叶片病斑、NO含量、NO亚细胞定位和木质部超微结构的影响。结果表明，病菌侵染导致了日本晴叶片呈现枯黄色的干枯斑，疣粒野生稻叶片呈现褐色的凋亡斑，而且野生稻的病斑长度要明显短于日本晴的病斑长度。接种白叶枯病菌后日本晴叶片内NO含量未见明显的变化，而野生稻叶片内NO含量则显著升高，并且大部分的NO定位于导管细胞壁内。进一步通过电镜观察，发现病菌侵染诱导了野生稻叶片导管细胞壁厚度的明显增加。基于这些结果，推测NO参与了疣粒野生稻对白叶枯病的抗性，其功能可能包括诱导导管细胞壁增厚，从而抑制病菌的进一步侵染。
疣粒野生稻高抗水稻白葉枯病，但是其具體的抗性機製目前仍不清楚。一氧化氮（ nitric oxide，NO）是一種重要的信號分子，在植物的抗病反應中起到瞭重要的作用，然而對于NO是否參與疣粒野生稻對水稻白葉枯病的抗性目前仍缺乏研究。以抗病的疣粒野生稻和感病的水稻品種日本晴為材料，研究瞭接種白葉枯病菌對葉片病斑、NO含量、NO亞細胞定位和木質部超微結構的影響。結果錶明，病菌侵染導緻瞭日本晴葉片呈現枯黃色的榦枯斑，疣粒野生稻葉片呈現褐色的凋亡斑，而且野生稻的病斑長度要明顯短于日本晴的病斑長度。接種白葉枯病菌後日本晴葉片內NO含量未見明顯的變化，而野生稻葉片內NO含量則顯著升高，併且大部分的NO定位于導管細胞壁內。進一步通過電鏡觀察，髮現病菌侵染誘導瞭野生稻葉片導管細胞壁厚度的明顯增加。基于這些結果，推測NO參與瞭疣粒野生稻對白葉枯病的抗性，其功能可能包括誘導導管細胞壁增厚，從而抑製病菌的進一步侵染。
우립야생도고항수도백협고병，단시기구체적항성궤제목전잉불청초。일양화담（ nitric oxide，NO）시일충중요적신호분자，재식물적항병반응중기도료중요적작용，연이대우NO시부삼여우립야생도대수도백협고병적항성목전잉결핍연구。이항병적우립야생도화감병적수도품충일본청위재료，연구료접충백협고병균대협편병반、NO함량、NO아세포정위화목질부초미결구적영향。결과표명，병균침염도치료일본청협편정현고황색적간고반，우립야생도협편정현갈색적조망반，이차야생도적병반장도요명현단우일본청적병반장도。접충백협고병균후일본청협편내NO함량미견명현적변화，이야생도협편내NO함량칙현저승고，병차대부분적NO정위우도관세포벽내。진일보통과전경관찰，발현병균침염유도료야생도협편도관세포벽후도적명현증가。기우저사결과，추측NO삼여료우립야생도대백협고병적항성，기공능가능포괄유도도관세포벽증후，종이억제병균적진일보침염。
Or yza meyeriana, a wild rice species , is highly resistant to rice bacterial blight (BB ) , but the resistance mechanism is still unknown.Nitric oxide ( NO) is an important signaling molecule that plays a pivotal role in plant disease resistance responses .However, little study has focused on whether NO is involved in BB resistance of O. meyeriana.In this study, the effects of BB pathogen inoculation on leaf lesion , NO content, NO subcellular location and xylem ultrastructure were studied in O.meyeriana and a susceptible rice cultivar Nipponbare .Pathogen infection caused yellow necrotic lesions in Nipponbare and brown apoptotic lesion in O.meyeriana, and the lesion length was much longer in Nipponbare than in the wild rice .No difference was found in NO content in Nipponbare after pathogen inoculation .However , NO content was prominently increased by pathogen inoculation in the wild rice , and it was further discovered that NO was largely accumulated in xylem cell wall .In addition, electron microscopic observations showed that BB pathogen inoculation induced the thickening of xylem cell wall in O.meyeriana but not in Nippon-bare.Based on these results , we suggested that NO played an important role in the BB resistance of O.me yeriana, and the role might involve the thickening of xylem cell wall that could inhibit further invasion of the pathogen .
