CAJ | 학술논문

在有机溶剂中利用固定化脂肪酶Novozym 435催化酯化反应以分离扁桃酸对映体,考察了反应时间、温度、摇床转速、加酶量、扁桃酸浓度及乙醇浓度对反应转化率和产物对映体过量值(eep)的影响.结果表明,在二氯乙烷中,拆分扁桃酸对映体的最佳条件为：加酶量40 mg,扁桃酸20 mM,乙醇80 mM,转速150 rpm,温度40°C.在此条件下,反应24h后,转化率为30%,产物中R-扁桃酸乙酯eep达到92%.
재유궤용제중이용고정화지방매Novozym 435최화지화반응이분리편도산대영체,고찰료반응시간、온도、요상전속、가매량、편도산농도급을순농도대반응전화솔화산물대영체과량치(eep)적영향.결과표명,재이록을완중,탁분편도산대영체적최가조건위：가매량40 mg,편도산20 mM,을순80 mM,전속150 rpm,온도40°C.재차조건하,반응24h후,전화솔위30%,산물중R-편도산을지eep체도92%.
The aim of this study is to resolute (R,S)-mandelic acid enantiomers in organic solvent using immobilized lipase Novozym 435. The enantiomers was esterified with ethanol. Conversion of mandelic acid and the enantiomeric excess of the product (eep) were used to estimate the reaction efficiency. Reaction conditions were optimized and determined to be: enzyme loading 40 mg, concentration of mandelic acid 20 mM, concentration of ethanol 80 mM, shaker speed 150 rpm, temperature 40 °C, time 24 h. Under the optimal conditions, the conversion was 30% after 24 h of reaction and an eep of 92% was obtained.