CAJ | 학술논문

目的：利用流式细胞仪分选人脂肪来源细胞（adipose derived cells, ADCs）软骨潜能亚群并初步检测其体外成软骨能力。方法采用酶消化法分离ADCs，以CD61为表面标志，通过流式细胞仪分选纯化，所得CD61+和CD61-两群细胞进行体外成软骨诱导，3周后进行软骨特异性细胞外基质二型胶原免疫荧光染色和软骨特异性基因SOX9和COL II定量PCR检测。结果采用CD61作为分选标志可通过流式细胞仪分选纯化获得两群细胞亚群。免疫荧光染色结果显示两群细胞经软骨诱导后均表达二型胶原。定量PCR结果表明CD61+组SOX9和COL II表达高于CD61-组（p<0.05）。结论CD61+ADCs具有较强体外软骨分化能力，CD61有望成为ADCs软骨潜能亚群体外分选标志。
목적：이용류식세포의분선인지방래원세포（adipose derived cells, ADCs）연골잠능아군병초보검측기체외성연골능력。방법채용매소화법분리ADCs，이CD61위표면표지，통과류식세포의분선순화，소득CD61+화CD61-량군세포진행체외성연골유도，3주후진행연골특이성세포외기질이형효원면역형광염색화연골특이성기인SOX9화COL II정량PCR검측。결과채용CD61작위분선표지가통과류식세포의분선순화획득량군세포아군。면역형광염색결과현시량군세포경연골유도후균표체이형효원。정량PCR결과표명CD61+조SOX9화COL II표체고우CD61-조（p<0.05）。결론CD61+ADCs구유교강체외연골분화능력，CD61유망성위ADCs연골잠능아군체외분선표지。
Objectives To sort out chondrogenic subpopulation of human adipose derived cell (ADCs) with flow cytome-try and preliminarily examine its in vitro chondrogenic potential. Methods ADCs were isolated by enzymes. CD61+ and CD61- subpopulations were sorted out with flow cytometry and chondrogenically induced in vitro. After 3-week induction, col-lagen II, the specific extracellular matrix of cartilage, was examined with immunofluorescent staining and SOX9 and COL II, the specific genes of chondrocyte, were checked with PCR. Results CD61 could be used as a surface marker for cell sorting of ADCs with flow cytometry. The results of immunofluorescent staining demonstrated that after chondrogenic induction, both of the two cell populations expressed collagen II. Quantitative PCR results showed that the expression of SOX9 and COL II were higher in CD61+group than in CD61- group (p<0.05). Conclusions CD61+ADCs had a stronger chondrogenic differentiation capacity than CD61- cells. CD61 could be used as a specific marker for purifying chondrogenic subpopulation of ADCs.