湖南理工学院学报（自然科学版）
湖南理工學院學報（自然科學版）
호남리공학원학보（자연과학판）
JOURNAL OF HUNAN INSTITUTE OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCE)
2013年
4期
51-53
,共3页
刘宇%唐东芳%彭德正%谢方%张娇飞%刘洋慧%朱政斌%易健民
劉宇%唐東芳%彭德正%謝方%張嬌飛%劉洋慧%硃政斌%易健民
류우%당동방%팽덕정%사방%장교비%류양혜%주정빈%역건민
血浆%多肽组%乙腈沉淀
血漿%多肽組%乙腈沉澱
혈장%다태조%을정침정
plasma%peptidome%acetonitrile precipitation
采用液相色谱-质谱联用技术，对血浆多肽组学样品进行前处理和优化，建立了血浆多肽组样品收集方法.通过考察乙腈沉淀和加热变性两种方法对血浆中蛋白质去除效率，确定采用乙腈沉淀；进一步对乙腈沉淀法进行优化，发现2.5倍体积乙腈沉淀达到最佳效果，处理的样本适合血浆多肽组学分析.
採用液相色譜-質譜聯用技術，對血漿多肽組學樣品進行前處理和優化，建立瞭血漿多肽組樣品收集方法.通過攷察乙腈沉澱和加熱變性兩種方法對血漿中蛋白質去除效率，確定採用乙腈沉澱；進一步對乙腈沉澱法進行優化，髮現2.5倍體積乙腈沉澱達到最佳效果，處理的樣本適閤血漿多肽組學分析.
채용액상색보-질보련용기술，대혈장다태조학양품진행전처리화우화，건립료혈장다태조양품수집방법.통과고찰을정침정화가열변성량충방법대혈장중단백질거제효솔，학정채용을정침정；진일보대을정침정법진행우화，발현2.5배체적을정침정체도최가효과，처리적양본괄합혈장다태조학분석.
Different methods for plasma peptidome preparation in peptidomics analysis were compared in this study. Plasma proteins were either precipitated with acetonitrile or subjected to themal denaturation. The eluent was subjected to analyze by Gel filtration high pressure liquid chromatography. The results indicated that acetonitrile could effectively precipitated plasma proteins, and had the best extracting effect. 2.5 fold acetonitrile was optimal. The results showed that the method was satisfactory and would be applied in peptidomics analysis of preparation.
