CAJ | 학술논문

目的：探讨大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）诱导为神经元样细胞后对大鼠脑损伤模型修复的影响。方法：骨髓贴壁法分离培养大鼠BMSCs并采用流式细胞术进行鉴定，川芎嗪诱导其分化，将诱导24 h的细胞直接注射移植到大鼠脑缺血模型海马区组织，检测分化的神经元样细胞在神经系统微环境中存活情况及病理变化。结果：细胞移植组病理变化与脑损伤模型组相比，神经细胞变性、坏死数量明显减少，间质水肿较轻。结论：骨髓间充质干细胞诱导为神经元样细胞后移植入对大鼠脑损伤的修复有一定的作用。
목적：탐토대서골수간충질간세포（bone marrow mesenchymal stem cells，BMSCs）유도위신경원양세포후대대서뇌손상모형수복적영향。방법：골수첩벽법분리배양대서BMSCs병채용류식세포술진행감정，천궁진유도기분화，장유도24 h적세포직접주사이식도대서뇌결혈모형해마구조직，검측분화적신경원양세포재신경계통미배경중존활정황급병리변화。결과：세포이식조병리변화여뇌손상모형조상비，신경세포변성、배사수량명현감소，간질수종교경。결론：골수간충질간세포유도위신경원양세포후이식입대대서뇌손상적수복유일정적작용。
Objective:To investigate the effect of neuron-like cells induced from bone marrow mesenchymal stem cells(BMSCs)in the repairment of brain injury in rats. Methods:BMSCs were isolated and cultured from rat’s bone marrow by adherent method,identified by flow cytometry. BMSCs were induced into neuron-like cells using ligustrazine. 24 hours after neuron-like cell induction,the cells were directly transplanted into the hippocampus of rats with brain injury,and the survival of neuron-like cells in microenvironment of nervous system and pathological changes was examined. Results:Compared to rats with brain injury alone,rats that received neuron-like cells transplantation showed a decreased number of nerve cell degeneration and necrosis and less significant interstitial edema. Conclusion:Neuron-like cells induced from BMSC can improve brain function of rats with brain injury.