CAJ | 학술논문

目的：建立稳定的大鼠糖尿病合并局灶性脑缺血损伤模型，并对有关建模成功的标准进行探讨与分析。方法：40只健康雄性SD大鼠随机分为正常饲养假手术组、正常饲养脑缺血再灌注损伤模型组、糖尿病脑缺血再灌注损伤模型组，糖尿病模型组一次性腹腔注射（ip）链脲佐菌素（streptozotocin，STZ）60 mg·kg-1建立1型糖尿病大鼠模型，分别在造模1，4周后检测各组大鼠的体质量和空腹血糖。在ip STZ 4周后采用线栓法制备大鼠局灶性脑缺血再灌注模型，缺血30 min，复灌注24 h，进行神经功能评分，测定脑梗死体积并计算梗死体积百分比。结果：腹腔注射STZ 4周后，糖尿病模型组与正常组大鼠相比，体质量显著下降（P<0.001），血糖值显著升高（P<0.001）。缺血复灌注损伤手术后，糖尿病模型组与正常组大鼠相比，再灌注损伤加重，脑梗死体积百分比显著增加（P<0.001）。模型成功率为73%。结论：本实验方法制备的大鼠糖尿病合并局灶性脑缺血损伤模型成功率较高，具有较好的重复性和稳定性。
목적：건립은정적대서당뇨병합병국조성뇌결혈손상모형，병대유관건모성공적표준진행탐토여분석。방법：40지건강웅성SD대서수궤분위정상사양가수술조、정상사양뇌결혈재관주손상모형조、당뇨병뇌결혈재관주손상모형조，당뇨병모형조일차성복강주사（ip）련뇨좌균소（streptozotocin，STZ）60 mg·kg-1건립1형당뇨병대서모형，분별재조모1，4주후검측각조대서적체질량화공복혈당。재ip STZ 4주후채용선전법제비대서국조성뇌결혈재관주모형，결혈30 min，복관주24 h，진행신경공능평분，측정뇌경사체적병계산경사체적백분비。결과：복강주사STZ 4주후，당뇨병모형조여정상조대서상비，체질량현저하강（P<0.001），혈당치현저승고（P<0.001）。결혈복관주손상수술후，당뇨병모형조여정상조대서상비，재관주손상가중，뇌경사체적백분비현저증가（P<0.001）。모형성공솔위73%。결론：본실험방법제비적대서당뇨병합병국조성뇌결혈손상모형성공솔교고，구유교호적중복성화은정성。
Objective:To establish a reliable model of focal cerebral ischemia injury in diabetic rats,and to explore the criteria for successful modeling. Methods: Forty healthy male SD rats were randomly divided into three groups: a normal sham-operation group,a normal cerebral ischemia-reperfusion injury group and a group of diabetes combined with cerebral ischemia-reperfusion injury. Type I diabetes was induced by an intraperitoneal injection of streptozotocin(STZ,60 mg·kg-1). The body mass and the level of fasting blood glucose in each group were measured on Weeks 1 and 4 after STZ injection. A focal cerebral ischemia-reperfusion model was prepared by a thread occlusion method four weeks after STZ treatment. The scores of neurological function were assessed and the percentage of infarct volume were calculated after 30 min ischemia followed by 24 h of reperfusion. Results: The body mass (P<0.001)was reduced and the fasting blood glucose(P<0.001)was increased significantly in diabetic rats than those in normal rats four weeks after STZ injection. The percentage of infarct volume(P<0.001)was increased more significantly and the brain injury appeared more severe in diabetic rats than in normal rats after reperfusion. The success rate of this model was 73%. Conclusion: In this study,we developed a model of focal cerebral ischemia injury in diabetic rats that demonstrates a high success rate and good reliability and stability.