中国神经精神疾病杂志
中國神經精神疾病雜誌
중국신경정신질병잡지
CHINESE JOURNAL OF NERVOUS AND MENTAL DISEASES
2013年
12期
710-714
,共5页
刘浏%谭关平%陈耀隆%詹傲%熊伟茗%何朝晖
劉瀏%譚關平%陳耀隆%詹傲%熊偉茗%何朝暉
류류%담관평%진요륭%첨오%웅위명%하조휘
铁调素%蛛网膜下腔出血%脑铁含量
鐵調素%蛛網膜下腔齣血%腦鐵含量
철조소%주망막하강출혈%뇌철함량
Hepcidin%Subarachnoid hemorrhage%Brain iron content
目的：观察大鼠蛛网膜下腔出血（subarachnoid hemorrhage，SAH）后早期海马铁含量及铁调素（Hep-cidin）蛋白的表达变化，探讨SAH后早期Hepcidin参与脑铁代谢紊乱的可能机制。方法90只成年雄性SD大鼠，随机分为假手术对照组和蛛网膜下腔出血组。采用视交叉前池单次注血法构建大鼠SAH模型。在SAH后早期12、24、48、72h采用免疫组化、Western blot法检测大鼠SAH后海马组织内Hepcidin的蛋白表达情况，采用原子吸收光谱仪测定海马组织总铁含量。结果免疫组化实验发现，与假手术对照组（0.19±0.05）比较，大鼠SAH后12h（0.30±0.06）见Hepcidin表达水平明显增高,至72h（0.56±0.07）呈逐渐增高趋势，差异有统计学意义（F=31.911，P﹤0.05）；Western blot实验同样发现，与假手术对照组（0.238±0.047）比较，大鼠SAH后12h（0.481±0.065）见Hepcidin蛋白表达水平明显增高,至72h（1.627±0.143）呈逐渐增高趋势，差异有统计学意义（F=147.314，P﹤0.05）；原子吸收光谱仪检测大鼠蛛网膜下腔出血后12h海马总铁含量（58.50±9.19）开始增加，至72h（99.34±7.68）呈逐渐增高趋势，与假手术对照组（43.51±4.59）比较，差异有统计学意义（F=28.799,P﹤0.05）；大鼠海马组织Hepcidin蛋白的表达量与海马铁含量成正相关（r=0.914，P<0.05）。结论大鼠SAH后脑铁含量的增加可能与Hepcidin表达增高有关。
目的：觀察大鼠蛛網膜下腔齣血（subarachnoid hemorrhage，SAH）後早期海馬鐵含量及鐵調素（Hep-cidin）蛋白的錶達變化，探討SAH後早期Hepcidin參與腦鐵代謝紊亂的可能機製。方法90隻成年雄性SD大鼠，隨機分為假手術對照組和蛛網膜下腔齣血組。採用視交扠前池單次註血法構建大鼠SAH模型。在SAH後早期12、24、48、72h採用免疫組化、Western blot法檢測大鼠SAH後海馬組織內Hepcidin的蛋白錶達情況，採用原子吸收光譜儀測定海馬組織總鐵含量。結果免疫組化實驗髮現，與假手術對照組（0.19±0.05）比較，大鼠SAH後12h（0.30±0.06）見Hepcidin錶達水平明顯增高,至72h（0.56±0.07）呈逐漸增高趨勢，差異有統計學意義（F=31.911，P﹤0.05）；Western blot實驗同樣髮現，與假手術對照組（0.238±0.047）比較，大鼠SAH後12h（0.481±0.065）見Hepcidin蛋白錶達水平明顯增高,至72h（1.627±0.143）呈逐漸增高趨勢，差異有統計學意義（F=147.314，P﹤0.05）；原子吸收光譜儀檢測大鼠蛛網膜下腔齣血後12h海馬總鐵含量（58.50±9.19）開始增加，至72h（99.34±7.68）呈逐漸增高趨勢，與假手術對照組（43.51±4.59）比較，差異有統計學意義（F=28.799,P﹤0.05）；大鼠海馬組織Hepcidin蛋白的錶達量與海馬鐵含量成正相關（r=0.914，P<0.05）。結論大鼠SAH後腦鐵含量的增加可能與Hepcidin錶達增高有關。
목적：관찰대서주망막하강출혈（subarachnoid hemorrhage，SAH）후조기해마철함량급철조소（Hep-cidin）단백적표체변화，탐토SAH후조기Hepcidin삼여뇌철대사문란적가능궤제。방법90지성년웅성SD대서，수궤분위가수술대조조화주망막하강출혈조。채용시교차전지단차주혈법구건대서SAH모형。재SAH후조기12、24、48、72h채용면역조화、Western blot법검측대서SAH후해마조직내Hepcidin적단백표체정황，채용원자흡수광보의측정해마조직총철함량。결과면역조화실험발현，여가수술대조조（0.19±0.05）비교，대서SAH후12h（0.30±0.06）견Hepcidin표체수평명현증고,지72h（0.56±0.07）정축점증고추세，차이유통계학의의（F=31.911，P﹤0.05）；Western blot실험동양발현，여가수술대조조（0.238±0.047）비교，대서SAH후12h（0.481±0.065）견Hepcidin단백표체수평명현증고,지72h（1.627±0.143）정축점증고추세，차이유통계학의의（F=147.314，P﹤0.05）；원자흡수광보의검측대서주망막하강출혈후12h해마총철함량（58.50±9.19）개시증가，지72h（99.34±7.68）정축점증고추세，여가수술대조조（43.51±4.59）비교，차이유통계학의의（F=28.799,P﹤0.05）；대서해마조직Hepcidin단백적표체량여해마철함량성정상관（r=0.914，P<0.05）。결론대서SAH후뇌철함량적증가가능여Hepcidin표체증고유관。
Objective To examine the iron content and the expression of hepcidin in early period after subarach-noid hemorrhage (SAH) in rats, and to explore the role of hepcidin in dysregulation of brain iron metabolism after SAH. Methods Totally 90 adult male SD rats were randomly divided into two groups:the sham-operation group and SAH group. The SAH model was established by single blood injection to prechiasmatic cistern. Immunohistochemical and Western Blotting were used to examine the expression of hepcidin at 12, 24, 48 and 72h after SAH. Meanwhile, Atomic Absorption Spectrometer was used to detect the iron content. Results Immunochemistry showed that hepcidin expression in rats in SAH group began to rise at 12 h(0.30±0.06)and gradually increased over time until 72 h(0.56±0.07)compared with the sham group(0.19±0.05). The expression of hepcidin was significantly higher in SAH group than in the sham group(F=31.911, P<0.05). Western blot showed that hepcidin expression in rats in SAH group began to rise at 12h(0.481±0.065) and gradually increased over time until 72h(1.627±0.143)Compared with the sham group(0.238±0.047). The expression of hepcidin was significantly higher in SAH group than in the sham group after SAH(F=147.314,P<0.05). Iron content in SAH group began to rise at 12h after SAH(58.50±9.19)and gradually increased until 72 h(99.34±7.68). The iron con-tents in SAH group were higher at every time points than those in sham group(43.51±4.59)(F=28.799,P﹤0.05). The ex-pression of hepcidin was correlated with the iron content in SAH group(r=0.914,P﹤0.01). Conclusion The increase in iron content following SAH is associated with the increased hepcidin expression.
