畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2013年
12期
9-14,17
,共7页
何江峰%刘红葵%陆振翔%赵萌莉
何江峰%劉紅葵%陸振翔%趙萌莉
하강봉%류홍규%륙진상%조맹리
小黑麦%淀粉%淀粉合成酶基因%盐胁迫
小黑麥%澱粉%澱粉閤成酶基因%鹽脅迫
소흑맥%정분%정분합성매기인%염협박
triticale%starch%starch synthetase gene%salinity stress
小黑麦是由小麦和黑麦杂交形成的第一个异源多倍体,它既继承了小麦的优质高产,又具备了黑麦抗逆性强的特点。主要利用实时定量PCR技术测定小黑麦淀粉合成相关基因及淀粉含量在不同发育时期、不同盐胁迫下的表达情况,旨在深入了解盐胁迫对小黑麦淀粉合成酶及淀粉含量的影响。研究发现:不同的淀粉合成酶基因在不同程度盐胁迫下其表达均有不同程度的变化,且淀粉含量变化与淀粉合成酶基因表达有着直接的相关性。该研究表明,盐胁迫可以改变淀粉组成及其性质。
小黑麥是由小麥和黑麥雜交形成的第一箇異源多倍體,它既繼承瞭小麥的優質高產,又具備瞭黑麥抗逆性彊的特點。主要利用實時定量PCR技術測定小黑麥澱粉閤成相關基因及澱粉含量在不同髮育時期、不同鹽脅迫下的錶達情況,旨在深入瞭解鹽脅迫對小黑麥澱粉閤成酶及澱粉含量的影響。研究髮現:不同的澱粉閤成酶基因在不同程度鹽脅迫下其錶達均有不同程度的變化,且澱粉含量變化與澱粉閤成酶基因錶達有著直接的相關性。該研究錶明,鹽脅迫可以改變澱粉組成及其性質。
소흑맥시유소맥화흑맥잡교형성적제일개이원다배체,타기계승료소맥적우질고산,우구비료흑맥항역성강적특점。주요이용실시정량PCR기술측정소흑맥정분합성상관기인급정분함량재불동발육시기、불동염협박하적표체정황,지재심입료해염협박대소흑맥정분합성매급정분함량적영향。연구발현:불동적정분합성매기인재불동정도염협박하기표체균유불동정도적변화,차정분함량변화여정분합성매기인표체유착직접적상관성。해연구표명,염협박가이개변정분조성급기성질。
Triticale was the first allopolyploidy that was formed by crossbreeding wheat and rye,which inherited the high yield and high quality of wheat and strong stress resistance. The expression situations of starch synthesis genes and starch content in triticale in different development stage and under different salinity stress were determined by the real-time PCR technology,so as to further understand the effects of salinity stress on starch synthetase gene and starch content. The results showed that the expression of different starch synthetase gene under different degree of salinity stress had different degree of variation and the changes of starch content had a direct correlation with the expression of starch synthetase gene. The study results showed that the salinity stress could change the composition and properties of starch.