CAJ | 학술논문

以吉富罗非鱼（Oreochromis niloticus）作为研究对象，采用室内水族箱模拟水生态系统，研究了20 d试验期内，水体中初始质量浓度为0.05 g L-1的三聚氰胺在罗非鱼肝脏中的累积规律及不同暴露时间，对罗非鱼肝脏中7-乙氧基-异酚噁唑酮-脱乙基酶（EROD）和谷胱甘肽-S-转移酶（GST）活性的影响。结果表明：随着试验时间的延长，三聚氰胺在罗非鱼肝脏中的质量分数先迅速上升，在第2天高达4.15 mg·kg-1。随后逐渐下降，在第20天降至0.8 mg·kg-1，恢复到暴露初期水平。与对照组相比，罗非鱼肝脏中EROD和GST的活性在第1天被轻微诱导之后，分别在第2天和第4天开始被显著抑制，抑制率分别为50.7%和69.4%。随后两者的活性上升并分别在第7天和第10天开始被显著诱导，诱导率各为122.9%和47.7%。总体上看，EROD和GST的活性皆呈现出诱导-抑制-诱导的变化趋势。但在暴露结束时，这两种酶的活性又显著低于对照组，抑制率分别为34.1%和20.3%。结果显示，罗非鱼肝脏中EROD酶和GST酶，在一定程度上可作为水体中三聚氰胺污染的生物监测指标。
이길부라비어（Oreochromis niloticus）작위연구대상，채용실내수족상모의수생태계통，연구료20 d시험기내，수체중초시질량농도위0.05 g L-1적삼취청알재라비어간장중적루적규률급불동폭로시간，대라비어간장중7-을양기-이분오서동-탈을기매（EROD）화곡광감태-S-전이매（GST）활성적영향。결과표명：수착시험시간적연장，삼취청알재라비어간장중적질량분수선신속상승，재제2천고체4.15 mg·kg-1。수후축점하강，재제20천강지0.8 mg·kg-1，회복도폭로초기수평。여대조조상비，라비어간장중EROD화GST적활성재제1천피경미유도지후，분별재제2천화제4천개시피현저억제，억제솔분별위50.7%화69.4%。수후량자적활성상승병분별재제7천화제10천개시피현저유도，유도솔각위122.9%화47.7%。총체상간，EROD화GST적활성개정현출유도-억제-유도적변화추세。단재폭로결속시，저량충매적활성우현저저우대조조，억제솔분별위34.1%화20.3%。결과현시，라비어간장중EROD매화GST매，재일정정도상가작위수체중삼취청알오염적생물감측지표。
The bioaccumulation of melamine in the liver of tilapia(Oreochromis niloticus)and its effect on the activities of 7-ethoxyresorufin-O-deethylase (EROD) and glutathione S-transferase(GST)at various exposure time were investigated with a stimulated aquatic micro-system, during a 20 days trial period with initial melamine concentration of 0.05 g·L-1 in water. It was found that melamine could be absorbed quickly in the liver at the initial exposure stage, and its concentration reached the highest value of 4.15 mg·kg-1 at the 2nd day of exposure. With the increase of exposure time, the melamine residue in the liver decreased gradually. After 20 treated days, the melamine concentration was reduced to 0.8 mg·kg-1, resuming the level at the early stage of exposure. The analytical results indicated that compared with the control group, the EROD and GST activities in the treated group were slightly induced at the first day exposure, then significantly inhibited by 50.7%for EROD at the 2nd day and 69.4%for GST at the 4th day, respectively. Thereafter, pronounced inducements were observed in EROD activity by 122.9%at the 7th day, and in GST activity by 47.7% at the 10th day. In general, the activities of both enzymes followed a change tendency of inducement, inhibition, and inducement again with an increase of exposure time. At the end of the test period, the EROD activity and GST activity revealed obvious decrease with inhibition rates of 34.1%and 20.3%respectively. The results in the present work showed that EROD and GST may act as biomarkers for indicating melamine pollution in aquatic environment.