生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2013年
12期
1887-1892
,共6页
罗茜%马宏瑞%朱超%花莉
囉茜%馬宏瑞%硃超%花莉
라천%마굉서%주초%화리
内源呼吸%外源呼吸%好氧活性污泥%微生物群落结构%PCR-DGGE
內源呼吸%外源呼吸%好氧活性汙泥%微生物群落結構%PCR-DGGE
내원호흡%외원호흡%호양활성오니%미생물군락결구%PCR-DGGE
endogenous respiration%exogenous respiration%activated sludge%bacterial community%PCR-DGGE
通过模拟好氧活性污泥实验，采用PCR-DGGE技术并监测不同时期的CODCr、脱氢酶和总蛋白质的质量浓度，研究在外源和内源呼吸模式下，微生物群落结构及相关生物活性的变化。结果表明：未添加碳源处理中 CODCr的质量浓度稳定在100 mg·L-1左右；脱氢酶的质量浓度从1μg·mL-1降低到接近0μg·mL-1；总蛋白的质量浓度从0.05 mg·mL-1上升至0.07 mg·mL-1后下降至0.01 mg·mL-1左右；同时，微生物群落结构变化不大。外加葡萄糖处理中CODCr的质量浓度从1000 mg·L-1下降并稳定在100 mg·L-1左右；脱氢酶的质量浓度从2μg·mL-1上升到5μg·mL-1；总蛋白的质量浓度均从0.05 mg·mL-1上升至0.07 mg·mL-1后下降至0.005 mg·mL-1左右；同时，微生物群落多样性呈现收敛趋势。内源和外源呼吸模式下的活性污泥中，变形菌门（ Proteobacteria ）都是绝对优势种群，其次是拟杆菌门（ Bacteroidetes ）。内源呼吸末期δ-变形菌门（Deltaproteobacteria）消失，外源呼吸末期硝化螺菌门（Nitrospirae）消失。
通過模擬好氧活性汙泥實驗，採用PCR-DGGE技術併鑑測不同時期的CODCr、脫氫酶和總蛋白質的質量濃度，研究在外源和內源呼吸模式下，微生物群落結構及相關生物活性的變化。結果錶明：未添加碳源處理中 CODCr的質量濃度穩定在100 mg·L-1左右；脫氫酶的質量濃度從1μg·mL-1降低到接近0μg·mL-1；總蛋白的質量濃度從0.05 mg·mL-1上升至0.07 mg·mL-1後下降至0.01 mg·mL-1左右；同時，微生物群落結構變化不大。外加葡萄糖處理中CODCr的質量濃度從1000 mg·L-1下降併穩定在100 mg·L-1左右；脫氫酶的質量濃度從2μg·mL-1上升到5μg·mL-1；總蛋白的質量濃度均從0.05 mg·mL-1上升至0.07 mg·mL-1後下降至0.005 mg·mL-1左右；同時，微生物群落多樣性呈現收斂趨勢。內源和外源呼吸模式下的活性汙泥中，變形菌門（ Proteobacteria ）都是絕對優勢種群，其次是擬桿菌門（ Bacteroidetes ）。內源呼吸末期δ-變形菌門（Deltaproteobacteria）消失，外源呼吸末期硝化螺菌門（Nitrospirae）消失。
통과모의호양활성오니실험，채용PCR-DGGE기술병감측불동시기적CODCr、탈경매화총단백질적질량농도，연구재외원화내원호흡모식하，미생물군락결구급상관생물활성적변화。결과표명：미첨가탄원처리중 CODCr적질량농도은정재100 mg·L-1좌우；탈경매적질량농도종1μg·mL-1강저도접근0μg·mL-1；총단백적질량농도종0.05 mg·mL-1상승지0.07 mg·mL-1후하강지0.01 mg·mL-1좌우；동시，미생물군락결구변화불대。외가포도당처리중CODCr적질량농도종1000 mg·L-1하강병은정재100 mg·L-1좌우；탈경매적질량농도종2μg·mL-1상승도5μg·mL-1；총단백적질량농도균종0.05 mg·mL-1상승지0.07 mg·mL-1후하강지0.005 mg·mL-1좌우；동시，미생물군락다양성정현수렴추세。내원화외원호흡모식하적활성오니중，변형균문（ Proteobacteria ）도시절대우세충군，기차시의간균문（ Bacteroidetes ）。내원호흡말기δ-변형균문（Deltaproteobacteria）소실，외원호흡말기초화라균문（Nitrospirae）소실。
PCR-DGGE based microbial community analysis combining the determination of the concentration of CODCr, dehydrogenase and total protein was conducted to investigate bacterial community succession and the change of bioactivity in activated sludge fed with or without glucose as carbon resource. Results showed that:in sludge without glucose, bacterial community structure was compariably stable; CODCr concentration was stable at 100 mg·L-1 during the whole incubation of 720 h;dehydrogenase concentration fell to nearly 0 μg·mL-1 from 1 μg·mL-1; amendment with glucose, which raised dehydrogenase concentration to 5μg·mL-1 from 2μg·mL-1, lowered bacterial diversity and caused convergent development on bacterial community structure. Total protein in sludge fed with and without glucose both rose rapidly from 0.05 mg·mL-1 to 0.07 mg·mL-1 at 72 h, then decreased to and kept at 0.01 mg·mL-1 and 0.005 mg·mL-1, respectively. Proteobacteria, following by Bacteroidetes, is the absolutely predominant habitant in sludge under both endogenous and exogenous respiration. Deltaproteobacteria fade away under endogenous respiration over time, while Nitrospirae disappeared under exogenous respiration.
