CAJ | 학술논문

[目的]探讨血管内皮抑素（Endostatin ，ES）对三阴性乳腺癌（TNBC）化疗增敏的作用。[方法]37℃条件下，体外培养TNBC的MDA-MB-231细胞，用不同浓度多烯紫杉醇单药和重组人ES（rh-ES）两药混合分别孵育T NBC细胞24 h、48 h ，用M T T法检测细胞生长抑制率。[结果]多烯紫杉醇单药不同浓度（0．1μg/mL、1μg/mL、10μg/mL、100μg/mL和1000μg/mL）对MDA-MB-231细胞24 h半数抑制浓度（IC50）分别为：-8．6、-9．4、-6．36、1．6、-7。联合使用 rh-ES （0．05μg/mL 恩度）和多烯紫杉醇不同浓度（0．1μg/mL、1μg/mL、10μg/mL、100μg/mL ，1000μg/mL多西紫杉醇）对MDA-MB-231细胞24 h IC50分别为：1．23、1．29、1．26、-0．22和0．01。[结论]提高浓度并不能增加多烯紫杉醇对MDA-MB-231细胞的抑制率。rh-ES可以增加低浓度多烯紫杉醇对TNBC的MDA-MB-231细胞的敏感性。
[목적]탐토혈관내피억소（Endostatin ，ES）대삼음성유선암（TNBC）화료증민적작용。[방법]37℃조건하，체외배양TNBC적MDA-MB-231세포，용불동농도다희자삼순단약화중조인ES（rh-ES）량약혼합분별부육T NBC세포24 h、48 h ，용M T T법검측세포생장억제솔。[결과]다희자삼순단약불동농도（0．1μg/mL、1μg/mL、10μg/mL、100μg/mL화1000μg/mL）대MDA-MB-231세포24 h반수억제농도（IC50）분별위：-8．6、-9．4、-6．36、1．6、-7。연합사용 rh-ES （0．05μg/mL 은도）화다희자삼순불동농도（0．1μg/mL、1μg/mL、10μg/mL、100μg/mL ，1000μg/mL다서자삼순）대MDA-MB-231세포24 h IC50분별위：1．23、1．29、1．26、-0．22화0．01。[결론]제고농도병불능증가다희자삼순대MDA-MB-231세포적억제솔。rh-ES가이증가저농도다희자삼순대TNBC적MDA-MB-231세포적민감성。
[Objective] To explore the effect of endostatin(ES) on chemotherapeutic sensitization of triple-negative breast cancer(TNBC) .[Methods] TNBC MDA-MB-231 cells were cultured at 37℃ ,and incubated with different concentration of only docetaxel or mixed with recombinant human ES for 24h and 48h .MTT as-say was used to detect the inhibition rate of cell growth .[Results] The inhibition rate of different concentra-tion of docetaxel(0 .1μg/mL ,1μg/mL ,10μg/mL ,100μg/mL and 1000μg/mL) on MDA-MB-231 cells were-8 .6 ,-9 .4 ,-6 .36 ,1 .6 and -7 ,respectively .The inhibition rate of ES (0 .05μg/mL) combined with dif-ferent concentration of docetaxel(0 .1μg/mL ,1μg/mL ,10μg/mL ,100μg/mL and 1000μg/mL) on MDA-MB-231 cells were 1 .23 ,1 .29 ,1 .26 ,-0 .22 and 0 .01 ,respectively .[Conclusion] Elevating the concentration can not increase the inhibition rate of docetaxel on MDA-MB-231 cells .Endostatin can increase the sensitivity of low concentration of docetaxel to TNBC MDA-MB-231 cells .