CAJ | 학술논문

[目的]探讨低分子肝素（LMWH）对裸鼠胃癌原位模型生长、转移及趋化因子SDF-1表达的影响。[方法]应用原位移植方法建立裸鼠胃癌原位模型，术后7d将裸鼠随机分为3组：对照组（生理盐水）、化疗组（5-氟尿嘧啶）、LMWH组（低分子肝素钙），分别每周注药2次，共5周，第6周处死动物，观察裸鼠胃癌原位模型肿瘤转移及生长情况。采用RT-PCR技术检测胃肿瘤组织SDF-1 mRNA表达水平。[结果]化疗组、LMWH组肿瘤体积较对照组明显缩小，差异有统计学意义（ P ＜0．05）；LMWH组胃癌原位模型肿瘤转移率（20％）低于对照组（70％），差异有统计学意义（P ＜0．05），而与化疗组之间差异无统计学意义（P ＞0．05）；LMWH组SDF-1 mRNA表达均较对照组和化疗组低，差异有统计学意义（ P ＜0．05）。[结论]LM-W H可抑制裸鼠胃癌原位模型的肿瘤生长及远处转移，其部分机制可能与降低肿瘤SDF-1的表达有关。
[목적]탐토저분자간소（LMWH）대라서위암원위모형생장、전이급추화인자SDF-1표체적영향。[방법]응용원위이식방법건립라서위암원위모형，술후7d장라서수궤분위3조：대조조（생리염수）、화료조（5-불뇨밀정）、LMWH조（저분자간소개），분별매주주약2차，공5주，제6주처사동물，관찰라서위암원위모형종류전이급생장정황。채용RT-PCR기술검측위종류조직SDF-1 mRNA표체수평。[결과]화료조、LMWH조종류체적교대조조명현축소，차이유통계학의의（ P ＜0．05）；LMWH조위암원위모형종류전이솔（20％）저우대조조（70％），차이유통계학의의（P ＜0．05），이여화료조지간차이무통계학의의（P ＞0．05）；LMWH조SDF-1 mRNA표체균교대조조화화료조저，차이유통계학의의（ P ＜0．05）。[결론]LM-W H가억제라서위암원위모형적종류생장급원처전이，기부분궤제가능여강저종류SDF-1적표체유관。
[Objective] To explore the effect of low-molecular weight heparin(LMWH) on tumor metasta-sis and grow th and the expression of SDF-1 in orthotopic transplantation gastric tumor model of nude mice .[Methods] The models of orthotopic transplantation gastric cancer in nude mice was established by orthotopic transplantation .All nude mice were randomly divided into control group (normal saline) ,chemotherapy group (5-fluorouracil) and LWMH group(LWMH) .The treatment duration was twice a week for 5 weeks .The ani-mals were sacrificed at 6 weeks .The tumor growth and metastasis of orthotopic gastric cancer in nude mice were observed .RT-PCR was used to detect the expression of SDF-1 mRNA in gastric cancer tissues .[Re-sults]Compared with control group ,the tumor size in chemotherapy group and LMWH group were reduced obviously ,and there was significant difference( P <0 .05) .The tumor metastasis rate in LMWH group was lower than that in control group(20% vs .70% ) ,and there was significant difference between LMWH group and control group( P<0 .05) ,but there was no difference between LMWH group and chemotherapy group( P>0 .05) .The expression of SDF-1 mRNA in LMWH group was lower than that in control group and chemo-therapy group ,and there was significant difference( P <0 .05) .[Conclusion] LMWH can inhibit the tumor growth and distant metastasis of orthotopic transplantation gastric cancer in nude mice .The mechanism may be associated with the decreasing of SDF-1 expression .