中国保健营养(中旬刊)
中國保健營養(中旬刊)
중국보건영양(중순간)
China Hwalth Care & nutrition
2013年
2期
180-181
,共2页
马一杰%赵卫杰%陈贝贝%陈小兵%李晓阳%罗素霞
馬一傑%趙衛傑%陳貝貝%陳小兵%李曉暘%囉素霞
마일걸%조위걸%진패패%진소병%리효양%라소하
结肠癌%miR-21%氟尿嘧啶%化疗药物敏感性
結腸癌%miR-21%氟尿嘧啶%化療藥物敏感性
결장암%miR-21%불뇨밀정%화료약물민감성
Colorectal cancer%MiR-21%5-FU%Chemo-sensitivity
目的:探讨反义 miR-21(as-miR-21)与结肠癌细胞对5-氟尿嘧啶(5-Fu)敏感性的关系。方法:分别应用5-FU、as-miR-21及两者同时作用于HT29大肠癌细胞株,通过干预试验与空白组进行对照,并采用MTT法及细胞形态观测的方法检测HT29大肠癌细胞株的凋亡情况。结果:同时干预as-miR-21及5-FU的试验组细胞凋亡增加,与分别单独应用5-FU或as-miR-21的实验组相比有统计学意义。结论:miR-21对结肠癌HT29细胞的增殖有抑制作用。同时抑制miR-21表达能增强5-Fu 的抑制大肠癌细胞增殖效果,这为以miR-21作为靶点的治疗手段用于临床治疗结肠癌提供理论依据。
目的:探討反義 miR-21(as-miR-21)與結腸癌細胞對5-氟尿嘧啶(5-Fu)敏感性的關繫。方法:分彆應用5-FU、as-miR-21及兩者同時作用于HT29大腸癌細胞株,通過榦預試驗與空白組進行對照,併採用MTT法及細胞形態觀測的方法檢測HT29大腸癌細胞株的凋亡情況。結果:同時榦預as-miR-21及5-FU的試驗組細胞凋亡增加,與分彆單獨應用5-FU或as-miR-21的實驗組相比有統計學意義。結論:miR-21對結腸癌HT29細胞的增殖有抑製作用。同時抑製miR-21錶達能增彊5-Fu 的抑製大腸癌細胞增殖效果,這為以miR-21作為靶點的治療手段用于臨床治療結腸癌提供理論依據。
목적:탐토반의 miR-21(as-miR-21)여결장암세포대5-불뇨밀정(5-Fu)민감성적관계。방법:분별응용5-FU、as-miR-21급량자동시작용우HT29대장암세포주,통과간예시험여공백조진행대조,병채용MTT법급세포형태관측적방법검측HT29대장암세포주적조망정황。결과:동시간예as-miR-21급5-FU적시험조세포조망증가,여분별단독응용5-FU혹as-miR-21적실험조상비유통계학의의。결론:miR-21대결장암HT29세포적증식유억제작용。동시억제miR-21표체능증강5-Fu 적억제대장암세포증식효과,저위이miR-21작위파점적치료수단용우림상치료결장암제공이론의거。
Objective:To study the effect ofas-miR-21 enhance HT29 colon cancer cell chemo-sensitivity to 5-FU. Methods:MTT assay and cell observation was applied to evaluate the sensitivity of HT29 colon cells to evaluate the effects of 5-FU、as-miR-21 or both of them.Results:Cell proliferation of the group which is affected by both 5-FU and as-miR-21 is significantly impressed. Conclusion: The expression of miR-21 could influnce the sensitivity of 5-FU chemotherapy, and may play an important role in the individualized treatment of CRC.