中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
578-581
,共4页
毛丹%张甦%陈钶%毛秀红%季申
毛丹%張甦%陳鈳%毛秀紅%季申
모단%장소%진아%모수홍%계신
中药%呕吐毒素%高效液相色谱-串联三重四极杆质谱%酸枣仁%淡豆豉%薏苡仁%补骨脂%麦芽
中藥%嘔吐毒素%高效液相色譜-串聯三重四極桿質譜%痠棘仁%淡豆豉%薏苡仁%補骨脂%麥芽
중약%구토독소%고효액상색보-천련삼중사겁간질보%산조인%담두시%의이인%보골지%맥아
Traditional Chinese herbal medicines%Deoxynivalenol%LC-MS-MS%Semen Ziziphi Spinosae%Semen Sojae Praepara-tum%Semen Coicis%Fructus Psoraleae%Malt Fructus
目的：建立酸枣仁、淡豆豉、薏苡仁、补骨脂等4种中药中呕吐毒素的高效液相色谱-串联三重四极杆质谱( LC-MS-MS)测定方法。方法：样品经水提取、免疫亲和柱净化后,用LC-MS-MS法进行分析测定。结果：呕吐毒素在2~50 ng·ml-1范围内进样量与峰面积呈良好的线性关系,r>0.999,加样回收率在68.7%~88.3%之间。结论：本法简便快速、灵敏度高、结果准确,可用于酸枣仁等4种中药中呕吐毒素的测定分析。
目的：建立痠棘仁、淡豆豉、薏苡仁、補骨脂等4種中藥中嘔吐毒素的高效液相色譜-串聯三重四極桿質譜( LC-MS-MS)測定方法。方法：樣品經水提取、免疫親和柱淨化後,用LC-MS-MS法進行分析測定。結果：嘔吐毒素在2~50 ng·ml-1範圍內進樣量與峰麵積呈良好的線性關繫,r>0.999,加樣迴收率在68.7%~88.3%之間。結論：本法簡便快速、靈敏度高、結果準確,可用于痠棘仁等4種中藥中嘔吐毒素的測定分析。
목적：건립산조인、담두시、의이인、보골지등4충중약중구토독소적고효액상색보-천련삼중사겁간질보( LC-MS-MS)측정방법。방법：양품경수제취、면역친화주정화후,용LC-MS-MS법진행분석측정。결과：구토독소재2~50 ng·ml-1범위내진양량여봉면적정량호적선성관계,r>0.999,가양회수솔재68.7%~88.3%지간。결론：본법간편쾌속、령민도고、결과준학,가용우산조인등4충중약중구토독소적측정분석。
Objective:To develop a method for determining deoxynivalenol in four kinds of traditional Chinese herbal medicines. Methods:After being extracted by water, purified by an immunoaffinity column, deoxynivalenol was analyzed by LC-MS-MS. Results:The calibration curve was linear within the range of 2-50 ng·ml-1 for deoxynivalenol. The recovery was 68. 7%-88. 3%. Conclusion:The method is simple, sensitive and accurate in the determination of deoxynivalenol in Semen Ziziphi Spinosae, Semen Sojae Praepara-tum, Semen Coicis and Fructus Psoraleae.
