CAJ | 학술논문

经过紫外扫描确定吡虫啉和啶虫脒的最大紫外吸收波长分别为270和246 nm，通过改变其比例优化出峰时间和分离度，确定V（乙腈）∶V（水）＝3∶7为流动相，建立了两种农药的标准曲线，结果表明在0.05～5μg· mL-1范围内线性关系均良好。进行样品加标回收实验，用乙腈提取，C18固相萃取柱净化，上述条件进行下分析，0.1，0.5，1.5 mg· kg-1三个加标浓度回收率均在75％～110％，变异系数＜5％，准确性和稳定性都较好，方法最低定量限0.01μg· g-1，完全可以用于实际样品的检测。
경과자외소묘학정필충람화정충미적최대자외흡수파장분별위270화246 nm，통과개변기비례우화출봉시간화분리도，학정V（을정）∶V（수）＝3∶7위류동상，건립료량충농약적표준곡선，결과표명재0.05～5μg· mL-1범위내선성관계균량호。진행양품가표회수실험，용을정제취，C18고상췌취주정화，상술조건진행하분석，0.1，0.5，1.5 mg· kg-1삼개가표농도회수솔균재75％～110％，변이계수＜5％，준학성화은정성도교호，방법최저정량한0.01μg· g-1，완전가이용우실제양품적검측。
The maximum absorption wavelength of imidacloprid and nitenpyram detected by UV spectrophotometer were 270 nm and 246 nm.To get optimum retention time and separation , the mobile phase should be acetonitrile and water ( V/V, 3∶7 ) .Standard curves of two pesticides were developed , which fit a linear regression ranging from 0.05 to 5 μg· mL-1 .After extracted by homogenate with acetonitrile and purified by C 18 SPE column, the recovery rates of the two pesticides were 75%~110% at the levels of 0.1mg· kg -1 , 0.5mg· kg-1 and 1.5mg· kg -1 with a RSD less than 5%, which showed a good accuracy and stability .This method with a limit of quantification ( LOQ) of 0.01μg· g-1 could be used in the practical detections .