CAJ | 학술논문

百合无症病毒（ Lily symptomless virus，LSV）是侵染百合科植物的主要病毒之一。根据该病毒不同分离株外壳蛋白基因（coat protein，CP）的保守序列，设计特异性引物与荧光探针，建立了LSV的实时荧光RT-PCR检测方法。利用该方法检测LSV的2个阳性样品，均能够得到典型扩增曲线，而在百合斑驳病毒、烟草环斑病毒、番茄环斑病毒等其他病毒阳性样品中没有典型的扩增曲线，表明了该方法的特异性。与普通RT-PCR法相比，此方法灵敏度提高10倍。应用该方法，2012年6月至今，宁波出入境检验检疫局从荷兰进境的67批百合种球样品中检出53批百合无症病毒阳性，检出率为93％。
백합무증병독（ Lily symptomless virus，LSV）시침염백합과식물적주요병독지일。근거해병독불동분리주외각단백기인（coat protein，CP）적보수서렬，설계특이성인물여형광탐침，건립료LSV적실시형광RT-PCR검측방법。이용해방법검측LSV적2개양성양품，균능구득도전형확증곡선，이재백합반박병독、연초배반병독、번가배반병독등기타병독양성양품중몰유전형적확증곡선，표명료해방법적특이성。여보통RT-PCR법상비，차방법령민도제고10배。응용해방법，2012년6월지금，저파출입경검험검역국종하란진경적67비백합충구양품중검출53비백합무증병독양성，검출솔위93％。
Lily symptomless virus (LSV) is one of the main viruses infecting Liliaceae plants.In the study, a real-time fluorescent RT-PCR method for LSV detection was established with specific primers and probe designed from the conservative sequences in coat protein genes of LSV isolates .Specificity study showed that typical amplification curves could be obtained for two LSV-positive samples , but not for Lily mottle virus, Tobacco ringspot virus and To-mato ringspot virus-positive samples .Sensitivity of this method was 10 times higher than that of regular RT-PCR. With this method, 53 batches of LSV were intercepted in 67 batches of lily imported from Holland since June 2012 by Ningbo Entry-Exit Inspection and Quarantine Bureau , and the detection rate was 93%.