CAJ | 학술논문

目的探讨过表达 Tribbles 同源蛋白3对小鼠成纤维细胞3T3-NIH 分泌胶原的影响。 　　方法使用腺病毒载体过量表达 Tribbles 同源蛋白3，使用 PCR 以及 Western blot 方法检测 Tribbles 同源蛋白3的过量表达情况，使用天狼猩红染色试剂盒以及 Western blot 检测胶原的表达。 　　结果使用过量表达 Tribbles 同源蛋白3的腺病毒载体可以增加成纤维细胞3T3-NIH 中 Tribbles 同源蛋白3的转录水平以及蛋白水平的表达，并增加3T3-NIH 细胞中 I型胶原的蛋白表达水平，降低 III 型胶原的蛋白表达水平。结论过量表达 Tribbles 同源蛋白3可以促进小鼠成纤维细胞 I 型胶原的表达与分泌。
목적탐토과표체 Tribbles 동원단백3대소서성섬유세포3T3-NIH 분비효원적영향。 　　방법사용선병독재체과량표체 Tribbles 동원단백3，사용 PCR 이급 Western blot 방법검측 Tribbles 동원단백3적과량표체정황，사용천랑성홍염색시제합이급 Western blot 검측효원적표체。 　　결과사용과량표체 Tribbles 동원단백3적선병독재체가이증가성섬유세포3T3-NIH 중 Tribbles 동원단백3적전록수평이급단백수평적표체，병증가3T3-NIH 세포중 I형효원적단백표체수평，강저 III 형효원적단백표체수평。결론과량표체 Tribbles 동원단백3가이촉진소서성섬유세포 I 형효원적표체여분비。
Objective The aim of this study is to investigate the effect on secretion of collagen with overexpression of tribbles homolog 3 (TRB3) in mouse fibroblast 3T3-NIH. Methods Using adenoviral vectors to overexpress TRB3, and the expression of TRB3 was detected by PCR and Western blot. The expression of collagen was detected by Sirius red staining kit and Western blot. Results The expression of TRB3 was significantly increased after infecting adenoviral vector. After overexpress TRB3 specifically, the expression and secretion of type I collagen were up-regulated in the mouse fibroblast cells, but the expression of type III collagen was decreased. Conclusion The overexpression of TRB3 promotes the secretion and expression of type I collagen in mouse fibroblasts cells.