饮料工业
飲料工業
음료공업
BEVERAGE INDUSTRY
2014年
1期
27-29
,共3页
赵贞%荆莹%李翠枝%刘丽君%邵建波
趙貞%荊瑩%李翠枝%劉麗君%邵建波
조정%형형%리취지%류려군%소건파
液相色谱法%合成色素%冷饮
液相色譜法%閤成色素%冷飲
액상색보법%합성색소%랭음
high performance liquid chromatography(HPLC)%synthetic pigment%cold drink
建立了冷饮中7种人工合成色素的高效液相色谱分析方法。样品用聚酰胺小柱吸附法提取色素,以甲醇-乙酸铵溶液为流动相梯度洗脱,通过C18反相色谱柱分离,紫外检测器245nm检测。结果表明柠檬黄、苋菜红、靛蓝、胭脂红、日落黄和诱惑红在0.3~100mg/kg,亮蓝在1~100mg/kg范围内呈良好的线性关系。7种色素的回收率为75.3%~102.7%。本方法用于冷饮中7种合成色素的测定,分离效果好,定量准确,重复性好。
建立瞭冷飲中7種人工閤成色素的高效液相色譜分析方法。樣品用聚酰胺小柱吸附法提取色素,以甲醇-乙痠銨溶液為流動相梯度洗脫,通過C18反相色譜柱分離,紫外檢測器245nm檢測。結果錶明檸檬黃、莧菜紅、靛藍、胭脂紅、日落黃和誘惑紅在0.3~100mg/kg,亮藍在1~100mg/kg範圍內呈良好的線性關繫。7種色素的迴收率為75.3%~102.7%。本方法用于冷飲中7種閤成色素的測定,分離效果好,定量準確,重複性好。
건립료랭음중7충인공합성색소적고효액상색보분석방법。양품용취선알소주흡부법제취색소,이갑순-을산안용액위류동상제도세탈,통과C18반상색보주분리,자외검측기245nm검측。결과표명저몽황、현채홍、전람、연지홍、일락황화유혹홍재0.3~100mg/kg,량람재1~100mg/kg범위내정량호적선성관계。7충색소적회수솔위75.3%~102.7%。본방법용우랭음중7충합성색소적측정,분리효과호,정량준학,중복성호。
A method was established for the determination of 7 synthetic pigments in cold drinks by high performance liq-uid chromatography (HPLC). Synthetic pigments in samples were extracted by a polyamide column and then separated on a C18 column with methanol and ammonium acetate as the mobile phase,and detected with a UV detector at 245nm. The re-sults showed that tartrazine,amaranth,indigo,carmine,sunset yellow and fancy red had a good linear relationship in the range of 0.3-100mg/kg,and that brilliant blue had the same situation in the range of 1-100mg/kg. The recovery rate of the 7 pigments was 75.3%-102.7%. The method showed good separation results and high accuracy and reproducibility when used for the determination of 7 synthetic pigments in cold drinks.