CAJ | 학술논문

背景：多项研究证实胚胎干细胞诱导的神经前体细胞能够促进脊髓损伤大鼠功能的恢复。 　　目的：观察胚胎干细胞经体外诱导培养的神经前体细胞在脊髓损伤大鼠中的作用。 　　方法：144只大鼠随机分为3组，实验组和对照组建立大鼠脊髓全横断模型，实验组造模后在椎管内损伤区上下两端注射胚胎干细胞衍生细胞；对照组于相同部位注射PBS；假手术组仅行椎板切除，不损伤脊髓，不做其他处理。 　　结果与结论：对照组和实验组大鼠造模后21 d后，对照组脊髓损伤区域转化生长因子β1表达显著高于实验组(P <0.05)。各时间点实验组大鼠脊髓中髓鞘碱性蛋白表达量显著高于对照组(P <0.05)。细胞移植后各时间点实验组BBB评分明显优于对照组(P <0.05)。提示胚胎干细胞培养诱导分化为神经前体细胞移植后期可降低转化生长因子β1的表达，并可以提高髓鞘碱性蛋白的表达变化，有助于大鼠全横断脊髓损伤的恢复。
배경：다항연구증실배태간세포유도적신경전체세포능구촉진척수손상대서공능적회복。 　　목적：관찰배태간세포경체외유도배양적신경전체세포재척수손상대서중적작용。 　　방법：144지대서수궤분위3조，실험조화대조조건립대서척수전횡단모형，실험조조모후재추관내손상구상하량단주사배태간세포연생세포；대조조우상동부위주사PBS；가수술조부행추판절제，불손상척수，불주기타처리。 　　결과여결론：대조조화실험조대서조모후21 d후，대조조척수손상구역전화생장인자β1표체현저고우실험조(P <0.05)。각시간점실험조대서척수중수초감성단백표체량현저고우대조조(P <0.05)。세포이식후각시간점실험조BBB평분명현우우대조조(P <0.05)。제시배태간세포배양유도분화위신경전체세포이식후기가강저전화생장인자β1적표체，병가이제고수초감성단백적표체변화，유조우대서전횡단척수손상적회복。
BACKGROUND:Several studies have demonstrated embryonic stem cells induced neural precursor cells can promote functional recovery in rats with spinal cord injury. OBJECTIVE:To study the effect of in vitro cultured embryonic stem cells induced neural precursor cells in rats with spinal cord injury. METHODS:Total y 144 rats were randomly divided into three groups. Experiment group and control group rats had spinal cord transection injury. Embryonic stem cells-derived cells were injected into the vertebral canal at rostral and caudal segment perilesional y for the experiment group whereas PBS solution was injected instead of cells in the control group. Sham surgery group rats had only laminectomy without any spinal cord injury and treatment. RESULTS AND CONCLUSION:The experimental result showed that at day 21 post-injury, the regional expression of transforming growth factor-β1 was greater in rats from the control group in comparison to the experiment group (P<0.05). At each time point after spinal cord injury in rats, the expression of myelin basic protein in the spinal cord was significantly higher in the experiment group than the control group (P<0.05). After celltransplantation, Basso, Beattie, and Bresnahan scores of the experiment group at different time points were significantly higher than those of the control group (P<0.05). Transplantation of in vitro cultured embryonic stem cells induced neural precursor cells can reduce the late expression of transforming growth factor-β1, and can increase the expression of myelin basic protein which contributes to the recovery of rats with completely transected spinal cord injury.