CAJ | 학술논문

背景：周围神经断伤后生长缓慢，失神经支配的肌肉萎缩及运动终板纤维化，导致肢体功能不可逆障碍。脐带间充质干细胞已经广泛应用于多学科研究，但应用于周围神经损伤中延缓大鼠失神经肌肉萎缩鲜有报道。目的：观察异种异基因脐带间充干细胞移植于大鼠离断坐骨神经断端，延缓失神经肌肉萎缩的效果。 　　方法：新鲜脐带采集于健康足月产妇，分离鉴定脐带间充质干细胞。制备大鼠坐骨神经SunderlandⅣ度损伤模型，去神经束5 mm，神经外膜修复，5 mm小间隙移植脐带间充质干细胞模型，对照组仅在小间隙内注入同体积生理盐水。测定大鼠坐骨神经功能指数，小腿三头肌湿质量，坐骨神经干潜伏期、动作电位传导速度、波幅，以及骨骼肌纤维横截面积维持率。 　　结果与结论：造模后4，8及12周，脐带间充质干细胞组大鼠坐骨神经功能指数、右侧小腿三头肌湿质量及骨骼肌纤维横截面积维持率均显著高于对照组(P <0.05)。造模后12周肌电图显示，脐带间充质干细胞组大鼠坐骨神经干潜伏期显著低于对照组，动作电位传导速度及波幅显著高于生理盐水对照组(P <0.001)。提示脐带间充质干细胞移植于大鼠离断的坐骨神经断端，可促进神经生长，延缓失神经肌肉萎缩，维持失神经肌肉形态及功能。
배경：주위신경단상후생장완만，실신경지배적기육위축급운동종판섬유화，도치지체공능불가역장애。제대간충질간세포이경엄범응용우다학과연구，단응용우주위신경손상중연완대서실신경기육위축선유보도。목적：관찰이충이기인제대간충간세포이식우대서리단좌골신경단단，연완실신경기육위축적효과。 　　방법：신선제대채집우건강족월산부，분리감정제대간충질간세포。제비대서좌골신경SunderlandⅣ도손상모형，거신경속5 mm，신경외막수복，5 mm소간극이식제대간충질간세포모형，대조조부재소간극내주입동체적생리염수。측정대서좌골신경공능지수，소퇴삼두기습질량，좌골신경간잠복기、동작전위전도속도、파폭，이급골격기섬유횡절면적유지솔。 　　결과여결론：조모후4，8급12주，제대간충질간세포조대서좌골신경공능지수、우측소퇴삼두기습질량급골격기섬유횡절면적유지솔균현저고우대조조(P <0.05)。조모후12주기전도현시，제대간충질간세포조대서좌골신경간잠복기현저저우대조조，동작전위전도속도급파폭현저고우생리염수대조조(P <0.001)。제시제대간충질간세포이식우대서리단적좌골신경단단，가촉진신경생장，연완실신경기육위축，유지실신경기육형태급공능。
BACKGROUND:Slow growth of peripheral nerve, muscle denervation atrophy and fossilization of motor end plate fol owing sciatic nerve injury cause irreversible limb function disorders. Umbilical cord mesenchymal stem cells have been widely used in multi-disciplinary research, but studies concerning umbilical cord mesenchymal stem cells delaying denervated muscle atrophy in rats fol owing peripheral nerve injury are rarely reported. OBJECTIVE:To observe the value of human umbilical cord mesenchymal stem cells transplantation to delay denervated muscle atrophy of rats after sciatic nerve injury. METHODS:Umbilical cord blood was col ected from healthy parturient woman after ful-term delivery. In the experimental group, the rat’s Sunderland IV degree sciatic nerve injury model was established by 5 mm denervation, epineurial repair, and 5 mm smal gap transplantation of umbilical cord mesenchymal stem cells. In the control group, after modeling, the same volume of normal saline was injected into the smal gap. The main outcome measures included the sciatic nerve function index, the wet weight of triceps surae,sciatic nerve latency, action potential conduction velocity and amplitude,and skeletal muscle fiber cross section area maintenance rate. RESULTS AND CONCLUSION:After 4, 8 and 12 weeks of modeling, the sciatic nerve function index values, the wet weight of triceps surae and skeletal muscle fiber cross section area maintenance rates in the experimental group were significantly higher than those in the control group (P<0.05). After 12 weeks of modeling, electromyography results showed sciatic nerve latency was significantly lower, but action potential conduction velocity and amplitude were dramatical y higher in the experimental group than the control group (P<0.05, P<0.001). Human umbilical cord mesenchymal stem cells transplantation in denervated muscle atrophy rats after sciatic nerve promotes nerve growth, delays denervated muscle atrophy, maintains the denervatied neuromusle’s morphology and function.