CAJ | 학술논문

背景：获得足够数量的细胞是细胞移植治疗中面临的一个关键问题，有研究表明可以通过合理的刺激促进干细胞增殖。 　　目的：观察还原型谷胱甘肽对人脐血间充质干细胞的生物学特性的影响。 　　方法：实验分为两组，对照组为正常人脐血间充质干细胞，实验组为使用0.15 g/L质量浓度的还原型谷胱甘肽处理的人脐血间充质干细胞。 　　结果与结论：MTT实验结果显示，干预后第5，7，9天，实验组细胞的吸光度值明显高于对照组(P<0.05)。流式细胞术显示，实验组还原型谷胱甘肽对人脐血间充质干细胞的表面标志分子 CD29\CD44\CD45\CD105的表达无影响。实时 PCR 结果显示，还原型谷胱甘肽可以促进人脐血间充质干细胞中细胞外信号调节激酶mRNA的表达(P<0.05)。结果证实，质量浓度0.15 g/L的还原型谷胱甘肽可促进人脐血干细胞增殖。
배경：획득족구수량적세포시세포이식치료중면림적일개관건문제，유연구표명가이통과합리적자격촉진간세포증식。 　　목적：관찰환원형곡광감태대인제혈간충질간세포적생물학특성적영향。 　　방법：실험분위량조，대조조위정상인제혈간충질간세포，실험조위사용0.15 g/L질량농도적환원형곡광감태처리적인제혈간충질간세포。 　　결과여결론：MTT실험결과현시，간예후제5，7，9천，실험조세포적흡광도치명현고우대조조(P<0.05)。류식세포술현시，실험조환원형곡광감태대인제혈간충질간세포적표면표지분자 CD29\CD44\CD45\CD105적표체무영향。실시 PCR 결과현시，환원형곡광감태가이촉진인제혈간충질간세포중세포외신호조절격매mRNA적표체(P<0.05)。결과증실，질량농도0.15 g/L적환원형곡광감태가촉진인제혈간세포증식。
BACKGROUND:How to obtain a sufficient number of cells is one of the key issues in the celltransplantation therapy, and studies have shown that stem cellproliferation can be promoted by reasonable stimulus. OBJECTIVE:To investigate reduced glutathione effects on biological characteristics of human umbilical cord blood mesenchymal stem cells. METHODS:The cells were divided into two groups:the control group consisted of the normal human umbilical cord blood mesenchymal stem cells, and in the experimental group, human umbilical cord blood mesenchymal stem cells were treated with 0.15 g/L reduced glutathione. RESULTS AND CONCLUSION:At days 5, 7, 9, cells treated with 0.15 g/L reduced glutathione showed higher absorbance values than those in the control group (P<0.05). Flow cytometry showed reduced glutathione had no effects on CD29, CD44, CD45, CD105 expression. Real-time PCR results showed reduced glutathione was capable of promoting extracellular signal-regulated kinase mRNA expression (P<0.05). Findings from this study showed that 0.15 g/L reduced glutathione can promote the proliferation of human umbilical cord blood mesenchymal stem cells.