中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
1期
27-32
,共6页
干细胞%骨髓干细胞%骨髓间充质干细胞%椎动脉型颈椎病%cathepsin B%白细胞介素6%肿瘤坏死因子α
榦細胞%骨髓榦細胞%骨髓間充質榦細胞%椎動脈型頸椎病%cathepsin B%白細胞介素6%腫瘤壞死因子α
간세포%골수간세포%골수간충질간세포%추동맥형경추병%cathepsin B%백세포개소6%종류배사인자α
bone marrow%mesenchymal stem cells%cervical vertebrae%interleukin-6%tumor necrosis factor-alpha%cathepsin B
背景：炎症刺激是椎动脉型颈椎病发病的重要因素，骨髓间充质干细胞具有很强的免疫调节作用，能有效抑制炎症，具有治疗椎动脉型颈椎病的潜能。<br> 目的：探讨椎动脉型颈椎病血管损伤机制及骨髓间充质干细胞的治疗作用。<br> 方法：40只日本大耳兔随机分为4组：空白组不作任何处理；模型组、丹参酮组、干细胞组采用硬化剂注射法制作椎动脉型颈椎病模型。造模24 h后，空白组，模型组不进行干预，丹参酮组和干细胞组经耳缘静脉注射丹参酮ⅡA磺酸钠溶液10 mL和骨髓间充质干细胞混悬液10 mL，干预2周后取材。<br> 结果与结论：与模型组相比，干细胞组管壁中膜平滑肌细胞肥大增生明显受到抑制，血管内皮皱折较匀称；干细胞组与丹参酮组比较无明显差别。与模型组相比，干细胞组椎动脉血管cathepsin B、白细胞介素6、肿瘤坏死因子α表达显著下降，差异有显著性意义(P <0.05)；模型组与丹参酮组比较差异无显著性意义(P >0.05)。结果可见炎症反应可能是椎动脉血管受到损伤的机制之一；骨髓间充质干细胞能有效抑制椎动脉血管炎症反应，修复受损椎动脉血管。
揹景：炎癥刺激是椎動脈型頸椎病髮病的重要因素，骨髓間充質榦細胞具有很彊的免疫調節作用，能有效抑製炎癥，具有治療椎動脈型頸椎病的潛能。<br> 目的：探討椎動脈型頸椎病血管損傷機製及骨髓間充質榦細胞的治療作用。<br> 方法：40隻日本大耳兔隨機分為4組：空白組不作任何處理；模型組、丹參酮組、榦細胞組採用硬化劑註射法製作椎動脈型頸椎病模型。造模24 h後，空白組，模型組不進行榦預，丹參酮組和榦細胞組經耳緣靜脈註射丹參酮ⅡA磺痠鈉溶液10 mL和骨髓間充質榦細胞混懸液10 mL，榦預2週後取材。<br> 結果與結論：與模型組相比，榦細胞組管壁中膜平滑肌細胞肥大增生明顯受到抑製，血管內皮皺摺較勻稱；榦細胞組與丹參酮組比較無明顯差彆。與模型組相比，榦細胞組椎動脈血管cathepsin B、白細胞介素6、腫瘤壞死因子α錶達顯著下降，差異有顯著性意義(P <0.05)；模型組與丹參酮組比較差異無顯著性意義(P >0.05)。結果可見炎癥反應可能是椎動脈血管受到損傷的機製之一；骨髓間充質榦細胞能有效抑製椎動脈血管炎癥反應，脩複受損椎動脈血管。
배경：염증자격시추동맥형경추병발병적중요인소，골수간충질간세포구유흔강적면역조절작용，능유효억제염증，구유치료추동맥형경추병적잠능。<br> 목적：탐토추동맥형경추병혈관손상궤제급골수간충질간세포적치료작용。<br> 방법：40지일본대이토수궤분위4조：공백조불작임하처리；모형조、단삼동조、간세포조채용경화제주사법제작추동맥형경추병모형。조모24 h후，공백조，모형조불진행간예，단삼동조화간세포조경이연정맥주사단삼동ⅡA광산납용액10 mL화골수간충질간세포혼현액10 mL，간예2주후취재。<br> 결과여결론：여모형조상비，간세포조관벽중막평활기세포비대증생명현수도억제，혈관내피추절교균칭；간세포조여단삼동조비교무명현차별。여모형조상비，간세포조추동맥혈관cathepsin B、백세포개소6、종류배사인자α표체현저하강，차이유현저성의의(P <0.05)；모형조여단삼동조비교차이무현저성의의(P >0.05)。결과가견염증반응가능시추동맥혈관수도손상적궤제지일；골수간충질간세포능유효억제추동맥혈관염증반응，수복수손추동맥혈관。
BACKGROUND:Inflammation is an important factor in cervical spondylotic vertebral arteriopathy, bone marrow mesenchymal stem cells have the potential to treat cervical spondylotic vertebral arteriopathy because of the immunomodulatory effects to inhibit inflammation. <br> OBJECTIVE:To investigate the injury mechanism of vascular injury in the model of cervical spondylotic vertebral arteriopathy and to study the therapeutic effects of bone marrow mesenchymal stem cells on it. <br> METHODS:Forty Japanese big ear rabbits were randomly divided into four groups:control group, model group, tanshinone group, and stem cellgroup. After modeling, the control and model groups were not given intervention, while the tanshinone and stem cellgroups were injected with tanshinone II A sodium sulfonate solution (10 mL) and bone marrow mesenchymal stem cellsuspension (10 mL) along the ear vein, respectively. After 2 weeks, the routine pathological examination was done to observe the vascular morphological changes, immunofluorescence staining was done to observe the cathepsin B expression in the vertebral artery, and ELISA was used to detect the expression of tumor necrosis factor-αand interleukin-6 in the vertebral artery. <br> RESULTS AND CONCLUSION:Compared with the model group, the arterial smooth muscle cellhypertrophy and hyperplasia was obviously restrained in stem cellgroup, and vascular endothelial fold was in symmetry, while no significant difference was found between stem cellgroup and tanshinone group. Compared with the model group, expressions of cathepsin B, interleukin 6 and tumor necrosis factor-αexpression were reduced significantly in the stem cellgroup (P<0.05), while there was no difference between the model and tanshinone groups (P>0.05). Inflammatory reaction may be one of mechanisms for vertebral artery damage, and bone marrow mesenchymal stem cells can effectively inhibit inflammation of the vertebral artery and improve vascular remodeling.
