CAJ | 학술논문

目的：探索用干粉状的肝浸粉、胰酪胨、月示胨代替传统破伤风杆菌产毒培养基中使用的猪肝水透析外液、胰酶酪蛋白消化液、浓胨水，以满足规模化生产的需求。方法与传统的破伤风杆菌产毒培养基同时用于破伤风类毒素的生产过程，由小批量生产逐步转化到规模化批量生产，经多批次试验，以检测不同组分制备的培养基各生化指标和接种细菌后培养的产毒水平进行比对。先确定肝浸粉可以取代猪肝水透析外液，在此基础上筛选出代替浓胨水的月示胨，再以待用的胰酪胨配合肝浸粉、月示胨制备多批次不同生产量的破伤风杆菌产毒培养基。结果经过多批次小批量扩大到批量规模化生产的试验，用肝浸粉、胰酪胨、月示胨配制的破伤风杆菌产毒培养基与传统工艺制备的破伤风杆菌产毒培养基相比较，无统计学意义（P＞0．05）。结论肝浸粉、胰酪胨、月示胨可以替代传统破伤风杆菌产毒培养基中的猪肝水透析外液、胰酶酪蛋白消化液、浓胨水，适用于破伤风类毒素的规模化生产。
목적：탐색용간분상적간침분、이락동、월시동대체전통파상풍간균산독배양기중사용적저간수투석외액、이매락단백소화액、농동수，이만족규모화생산적수구。방법여전통적파상풍간균산독배양기동시용우파상풍류독소적생산과정，유소비량생산축보전화도규모화비량생산，경다비차시험，이검측불동조분제비적배양기각생화지표화접충세균후배양적산독수평진행비대。선학정간침분가이취대저간수투석외액，재차기출상사선출대체농동수적월시동，재이대용적이락동배합간침분、월시동제비다비차불동생산량적파상풍간균산독배양기。결과경과다비차소비량확대도비량규모화생산적시험，용간침분、이락동、월시동배제적파상풍간균산독배양기여전통공예제비적파상풍간균산독배양기상비교，무통계학의의（P＞0．05）。결론간침분、이락동、월시동가이체대전통파상풍간균산독배양기중적저간수투석외액、이매락단백소화액、농동수，괄용우파상풍류독소적규모화생산。
Objective To explore using dry powdered liver extract powder , trypticase , and peptone as main materials , it is the need for large-scale production , preparation was carried out for the medium production of tetanus toxin instead of the traditional medium contained liver extracellular fluid , trypsin digestion of casein and concentrated peptone water . Methods The prepared midea and the traditional media were both used in production process of tetanus toxin , from a sim-ple procedure to a large scale gradually , different components of the culture medium were changed by the multi -batch exper-iments and inoculate bacterial seeds , and cultured toxin production levels were obtained and compared , so did for index of biochemical standard .Firstly, on the basis of the replacement of liver extract powder to the extra dialysis fluid , then selec-tion peptone to replace concentrated peptone water was carried out .In combination of these three materials in a toxin pro-duction medium , comparative tests were performed in both of media .Results Several tests are completed , from a small capacity to a production process , the biochemistry indexes are obtained in media after cultivation of bacteria .In preparation of toxin, the medium contained liver extract powder , trypticase and peptone was compared to the traditional medium , it has no statistical significant difference(P>0.05)in both media.Conclusions The liver extract powder, trypticase, and pep-tone can replace the liver extra-dialysis fluid, trypsin digestion of casein and concentrated peptone water in the traditional medium in preparation of tetanus toxin in an industrial production .