CAJ | 학술논문

目的：利用流式细胞技术检测耐多药结核分枝杆菌（ Multidrug resistant mycobacterium tuberculosis ， MDR-TB）临床分离株和结核分枝杆菌一、二线药物敏感株分别感染U937细胞后的凋亡率及其时相性变化。方法用两组细菌分别感染U937细胞，在感染后2、4、8及12 h用流式细胞技术检测各组感染U937细胞的凋亡率，并分析其时相性变化。结果结核分枝杆菌MDR株和敏感株均能诱导U937细胞凋亡，两组细胞凋亡率比较差异有统计学意义（P＜0．01），MDR株诱导U937细胞凋亡率显著低于敏感株诱导的凋亡率，且在诱导后2、4、8及12 h各时间点的凋亡率都显著低于敏感株组，诱导早期凋亡率升高显著，特别是诱导后2 h凋亡率升高最显著，4 h以后凋亡率升高逐渐变慢。结论结核分枝杆菌临床分离株感染U937细胞后能快速诱导其凋亡，凋亡率与诱导菌株的耐药表型相关，敏感株诱导的凋亡率显著高于MDR株，诱导早期凋亡率升高最显著，4 h以后凋亡率升高减慢。
목적：이용류식세포기술검측내다약결핵분지간균（ Multidrug resistant mycobacterium tuberculosis ， MDR-TB）림상분리주화결핵분지간균일、이선약물민감주분별감염U937세포후적조망솔급기시상성변화。방법용량조세균분별감염U937세포，재감염후2、4、8급12 h용류식세포기술검측각조감염U937세포적조망솔，병분석기시상성변화。결과결핵분지간균MDR주화민감주균능유도U937세포조망，량조세포조망솔비교차이유통계학의의（P＜0．01），MDR주유도U937세포조망솔현저저우민감주유도적조망솔，차재유도후2、4、8급12 h각시간점적조망솔도현저저우민감주조，유도조기조망솔승고현저，특별시유도후2 h조망솔승고최현저，4 h이후조망솔승고축점변만。결론결핵분지간균림상분리주감염U937세포후능쾌속유도기조망，조망솔여유도균주적내약표형상관，민감주유도적조망솔현저고우MDR주，유도조기조망솔승고최현저，4 h이후조망솔승고감만。
Objective To compare the apoptosis rate of macrophages U 937 infected with clinical isolates of MDR-TB and susceptible Mycobacterium tuberculosis by using a flow cytometry .Methods Macrophages U937 were respectively infected with MDR-TB and susceptible clinical isolates , then the apoptosis rate of macrophages U 937 were detected by the flow cy-tometry after infection in 2, 4, 8 and 12 hs and the phase changes of apoptosis rate of infected macrophages U 937 were ana-lyzed.Results U937 apoptosis can be induced both by the MDR-TB and the susceptible isolates , but the differences are significant in the apoptosis rates in between two types of isolates (P<0.01).The apoptosis rates of U937 infected with MDR-TB are significantly lower than that with susceptible isolates , and all of the apoptosis rates of U 937 after infection in 2, 4, 8 and 12 hs with MDR-TB are significantly lower than that with susceptible isolates .The apoptosis rates of U937 in-crease markedly in early stage of infection , especially after infection in 2 hs.The apoptosis rates slowly rise down after in-fection in 4 hs.Conclusion The macrophage U937 apoptosis rapidly occurrs after infecting with the isolates of mycobacte-rium tuberculosis.The apoptosis rates of U937 are correlated to the isolates of MDR-TB, and are significantly higher in sus-ceptible isolates than these in MDR-TB isolates, but rise slowly down after infection in 4 hs.