CAJ | 학술논문

建立高效液相色谱法测定大麦虫体内磷脂酰胆碱含量的方法。样品经甲醇提取、净化后，经高效液相色谱分析。采用外标法定量，磷脂酸胆碱标准溶液质量浓度在250～2000μg/mL范围内呈良好的线性相关，相关系数为0．9996，样品加标回收率在97％～109％之间，相对标准偏差小于2．4％。对不同生长阶段大麦虫体内磷脂酰胆碱含量测定结果表明，大麦虫体内卵磷脂含量随个体重量的变化符合二项式曲线的变化规律，相关系数R2＝0．865（P＜0．01）。
건립고효액상색보법측정대맥충체내린지선담감함량적방법。양품경갑순제취、정화후，경고효액상색보분석。채용외표법정량，린지산담감표준용액질량농도재250～2000μg/mL범위내정량호적선성상관，상관계수위0．9996，양품가표회수솔재97％～109％지간，상대표준편차소우2．4％。대불동생장계단대맥충체내린지선담감함량측정결과표명，대맥충체내란린지함량수개체중량적변화부합이항식곡선적변화규률，상관계수R2＝0．865（P＜0．01）。
A liquid chromatographic method for determination of phosphatidylcholine in Zophobsmorio L. has been developed. The samples were analyzed by liquid chromatography. Quantitative method was external standard method. The linearity was checked by standard solutions at concentration range from 250~2 000 μg/mL, the correlation coefficient was 0.999 6. The recovery values ranged from 97% to 109% with relative standard deviations ( RSDs) no larger than 2. 4%. For different growth stage of Zophobsmorio L., the results of the content of phosphatidylcholine in vivo showed that the content of phosphatidylcholine was in line with the binomial curve along with the change of the weight, the correlation coefficient R2 was 0.865 (P<0.01).