生物技术进展
生物技術進展
생물기술진전
2014年
1期
40-43
,共4页
孙菲%郑榕%杨煌建%张祝兰
孫菲%鄭榕%楊煌建%張祝蘭
손비%정용%양황건%장축란
胰脂肪酶%抑制剂%筛选模型%蛋白酶%淀粉酶
胰脂肪酶%抑製劑%篩選模型%蛋白酶%澱粉酶
이지방매%억제제%사선모형%단백매%정분매
pancrelipase%inhibitor%screening model%protease%amylase
本研究采用胰脂肪酶抑制剂筛选模型结合淀粉酶、蛋白酶抑制活性的测定，对来源于土壤的微生物进行高通量筛选，得到三株胰脂肪酶抑制剂产生菌FIM-1013、FIL-19和FIMe-3，其发酵产物对胰脂肪酶活性的抑制率分别为61％、53％和42％，对淀粉酶活性的抑制率分别为0、7％和3％，对蛋白酶活性的抑制率分别为6％、4％和0。结果显示，筛选所得的三个菌株均能产生具有选择性的胰脂肪酶抑制剂，值得进一步的研究。
本研究採用胰脂肪酶抑製劑篩選模型結閤澱粉酶、蛋白酶抑製活性的測定，對來源于土壤的微生物進行高通量篩選，得到三株胰脂肪酶抑製劑產生菌FIM-1013、FIL-19和FIMe-3，其髮酵產物對胰脂肪酶活性的抑製率分彆為61％、53％和42％，對澱粉酶活性的抑製率分彆為0、7％和3％，對蛋白酶活性的抑製率分彆為6％、4％和0。結果顯示，篩選所得的三箇菌株均能產生具有選擇性的胰脂肪酶抑製劑，值得進一步的研究。
본연구채용이지방매억제제사선모형결합정분매、단백매억제활성적측정，대래원우토양적미생물진행고통량사선，득도삼주이지방매억제제산생균FIM-1013、FIL-19화FIMe-3，기발효산물대이지방매활성적억제솔분별위61％、53％화42％，대정분매활성적억제솔분별위0、7％화3％，대단백매활성적억제솔분별위6％、4％화0。결과현시，사선소득적삼개균주균능산생구유선택성적이지방매억제제，치득진일보적연구。
A pancreatic lipase inhibitor screening model was constructed, which combined with determination of inhibitory activities of amylase, protease and lipase. Three strains FIM-1013, FIL-19 and FIMe-3 were obtained through target screening of microbial broths from soil organisms by this model. The lipase inhibitory activites of FIM-1013,FIL-19 and FIMe-3 were 61%, 53% and 42%, the amylase inhibitory activites were 0,7% and 3%, and the inhibitory protease activity were 6%,4% and 0. The results indicated that the three strains could produce very potent selective inhibitor of pancreatic lipase which deserved further research.
