生物技术进展
生物技術進展
생물기술진전
2014年
1期
30-35
,共6页
吴斌%郭芹%王刚霞%陈维信%李雪萍
吳斌%郭芹%王剛霞%陳維信%李雪萍
오빈%곽근%왕강하%진유신%리설평
一氧化氮%香蕉%软化%多聚半乳糖醛酸酶%基因
一氧化氮%香蕉%軟化%多聚半乳糖醛痠酶%基因
일양화담%향초%연화%다취반유당철산매%기인
nitric oxide%banana%softening%polygalacturonase%gene
为了解多聚半乳糖醛酸酶(PG)在香蕉采后软化中的分子调控机制,采用40μL/L NO熏蒸处理绿熟期的‘巴西’香蕉果实3 h后,在20℃和相对湿度为85%的条件下贮藏,研究NO对香蕉果实乙烯释放量、硬度、PG活性及MaPGs基因表达的影响。结果表明:NO 处理降低了果实乙烯释放量,延缓了果实硬度的下降,抑制了 PG 的活性;降低了MaPG2、MaPG3和MaPG4基因的表达,延缓了香蕉果实的软化。
為瞭解多聚半乳糖醛痠酶(PG)在香蕉採後軟化中的分子調控機製,採用40μL/L NO熏蒸處理綠熟期的‘巴西’香蕉果實3 h後,在20℃和相對濕度為85%的條件下貯藏,研究NO對香蕉果實乙烯釋放量、硬度、PG活性及MaPGs基因錶達的影響。結果錶明:NO 處理降低瞭果實乙烯釋放量,延緩瞭果實硬度的下降,抑製瞭 PG 的活性;降低瞭MaPG2、MaPG3和MaPG4基因的錶達,延緩瞭香蕉果實的軟化。
위료해다취반유당철산매(PG)재향초채후연화중적분자조공궤제,채용40μL/L NO훈증처리록숙기적‘파서’향초과실3 h후,재20℃화상대습도위85%적조건하저장,연구NO대향초과실을희석방량、경도、PG활성급MaPGs기인표체적영향。결과표명:NO 처리강저료과실을희석방량,연완료과실경도적하강,억제료 PG 적활성;강저료MaPG2、MaPG3화MaPG4기인적표체,연완료향초과실적연화。
In order to explore the molecular regulation mechanism of polygalacturonase ( PG) during banana softening, fruit harvested at the mature green stage were fumigated with 40 μL/L of NO for 3 h, and then stored at 20℃ with 85% relative humility. Ethylene production, firmness, PG activity and MaPGs genes were measured during banana ripening. The results showed that NO treatment resulted in significantly lower rates of ethylene production than those in the control fruit. The NO treated fruit underwent a lesser loss of firmness during storage. The activity of PG and expression of MaPG2、MaPG3 and MaPG4 genes were effectively reduced in NO treated fruit, indicating that the softening of banana fruit was delayed by NO treatment.