CAJ | 학술논문

目的：研究CBLB502对放射性肺炎和放射性肺纤维化的防护作用，从而证实CBLB502作为临床抗放射药物的可行性。方法 C57BL/6J小鼠20 Gy单次照射后24 h、1个月、3个月和5个月取肺组织用TUNEL方法对肺泡上皮细胞和血管内皮细胞的凋亡进行检测，HE染色观察纤维化病理变化，免疫组织化学方法检测特异性指标表达，以及受照部位皮毛和皮肤的病理变化。结果 CBLB502抑制了照射后小鼠肺泡上皮细胞和血管内皮细胞的凋亡，减缓了肺纤维化进程，同时减少层黏连蛋白表达，维持表面活性蛋白B表达量，受照皮肤的炎症反应也明显减轻。结论 CBLB502对照射后放射性肺炎和放射性肺纤维化的发生和进程有防护作用。
목적：연구CBLB502대방사성폐염화방사성폐섬유화적방호작용，종이증실CBLB502작위림상항방사약물적가행성。방법 C57BL/6J소서20 Gy단차조사후24 h、1개월、3개월화5개월취폐조직용TUNEL방법대폐포상피세포화혈관내피세포적조망진행검측，HE염색관찰섬유화병리변화，면역조직화학방법검측특이성지표표체，이급수조부위피모화피부적병리변화。결과 CBLB502억제료조사후소서폐포상피세포화혈관내피세포적조망，감완료폐섬유화진정，동시감소층점련단백표체，유지표면활성단백B표체량，수조피부적염증반응야명현감경。결론 CBLB502대조사후방사성폐염화방사성폐섬유화적발생화진정유방호작용。
Objective To investigate the protective effect of CBLB 502 on radiation pneumonitis and pulmonary fibrosis for confirming the feasibility of CBLB502 as a clinical anti-radiation drug release.Methods With a single 20 Gy irradia-tion, C57BL/6J mice was sacrificed on 24 h, 1 month, 3 months and 5 months and lung tissue was assayed by TUNEL method for apoptosis of alveolar epithelial cells and endothelial cells , HE staining showing fibrosis changes , immunohisto-chemistry detecting the expression of specific indicators , as well as pathological changes of the fur and skin radiated site . Results CBLB502 inhibits apoptosis in mice alveolar epithelial cells and vascular endothelial cells after irradiation , slowing the process of pulmonary fibrosis , while reducing the expression of laminin and maintaining the expression of surfac-tant protein B, and the skin inflammation also significantly reduced .Conclusion CBLB502 could alleviate the occurrence of radiation pneumonitis and pulmonary fibrosis as well as radiation-induced skin injury .